Nov 1, 1996

Induction of sialyl Lewis(X) on the surface of cultured rat vascular endothelial cells and cardiac myocytes by hypoxia/reoxygenation in vitro

The Journal of Pathology
Y SekoY Yazaki


Neutrophils adhere to and roll on vascular endothelial cells (VECs) through interaction of selections and their carbohydrate ligands in the early stages of inflammation; this adhesion is then later strengthened through interaction of integrins on neutrophils with intercellular adhesion molecule-1 (ICAM-1) on endothelial cells. Recent, as yet unpublished studies showed that myocardial ischaemia/reperfusion caused rapid expression of sialyl Lewis(X) (SLe(X)), one of the carbohydrate ligands of selections, on VECs and cardiac myocytes and that an anti-SLe(X) monoclonal antibody (MAb) significantly reduced myocardial reperfusion injury in vivo, In the present study, to investigate whether or not ischaemia/reperfusion itself can induce the expression of SLe(X) on VECs and cardiac myocytes, the expression of SLe(X) on cultured rat VECs and cardiac myocytes was examined by treatment with hypoxia/reoxygenation in vitro, because ischaemia/ reperfusion stimuli may partly be due to hypoxia/reoxygenation. The expression of SLe(X) was induced rapidly and temporarily on the surface of cultured rat cardiac myocytes and VECs by hypoxia/reoxygenation in vitro. This strongly suggests that the expression of SLeX on the surface of myocardial cells...Continue Reading

  • References15
  • Citations2

Mentioned in this Paper

Monoclonal Antibodies
Vascular Endothelial Cells
Cell Culture Techniques
Neutrophils as Percentage of Blood Leukocytes (Lab Test)
Intercellular adhesion molecule 1
Myocardial Ischemia
Coronary Arteriosclerosis

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