Induction of similar features of apoptosis in human and bovine vascular endothelial cells treated by 7-ketocholesterol

The Journal of Pathology
Gérard LizardL Lagrost

Abstract

Cholesterol oxides have numerous cytotoxic effects and those oxidized in the C7 position have been shown to induce apoptosis in bovine aortic endothelial cells (BAEC). The aim of the present study was to determine whether apoptosis also occurs in human vascular endothelial cells (HUVEC) treated with 7-ketocholesterol. To this end, cultured BAEC and HUVEC were incubated for 48 h with 7-ketocholesterol (concentration range 5-80 micrograms/ml) and the characteristics of cell death were assessed by various methods: counting of adherent and non-adherent cells; analysis of DNA fragmentation pattern; and morphological study by light, fluorescence, and electron microscopy. The 7-ketocholesterol treatment was accompanied by a decrease in the number of adherent cells and an increase in the number of non-adherent cells. Apoptotic cells, recognized by fragmented and/or condensed nuclei after staining with Hoechst 33342 or Giemsa, were mainly detected among non-adherent cells, and agarose gel electrophoresis revealed a typical internucleosomal DNA fragmentation among 7-ketocholesterol-treated cells. The DNA fragmentation was no longer detected when HUVEC and BAEC were simultaneously incubated with 0.5 mmol/l zinc chloride, which is known to...Continue Reading

References

Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
Jan 1, 1989·Free Radical Biology & Medicine·L L Smith, B H Johnson
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Jan 1, 1971·Annals of Internal Medicine·W B KannelP M McNamara
May 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J R HesslerG M Chisolm
May 1, 1995·Experimental Cell Research·E BonfocoP Nicotera
Jul 1, 1995·American Journal of Hematology·B ZhaoR Dierichs
Jul 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H HughesJ R Guyton
Mar 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·S Ayala-TorresE B Thompson
Apr 7, 1993·Biochimica Et Biophysica Acta·K L CarpenterM J Mitchinson
Nov 1, 1995·Atherosclerosis·K ClareM J Mitchinson
Jun 14, 1996·Biochemical and Biophysical Research Communications·E NishioY Watanabe
Jan 1, 1996·Free Radical Biology & Medicine·J A Berliner, J W Heinecke
Nov 1, 1995·Immunobiology·K AupeixP Bischoff
Jul 1, 1996·The Journal of Pathology·S J HardwickJ N Skepper
Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E W Raines, R Ross

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Citations

Mar 9, 2002·Molecular and Cellular Biology·Sudesh AgrawalGuy M Chisolm
Feb 14, 2009·Journal of Experimental Botany·Matthias Josef AffenzellerUrsula Lütz-Meindl
Jul 15, 2006·Endothelium : Journal of Endothelial Cell Research·LiChun ZhouHong Yang
Mar 26, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jean-Marie ZahmGérard Lizard
Apr 3, 2003·Biochemical and Biophysical Research Communications·Serge MonierGérard Lizard
Feb 22, 2005·Redox Report : Communications in Free Radical Research·Makoto ShimozawaToshikazu Yoshikawa
Feb 17, 2015·Steroids·Rodolphe FilomenkoSégolène Gambert-Nicot
May 3, 2014·Journal of Toxicology and Environmental Health. Part a·Yunfeng ZongXin Zhang
Aug 23, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A VejuxG Lizard
May 4, 1999·The International Journal of Biochemistry & Cell Biology·M A Lyons, A J Brown
Apr 25, 2007·Current Eye Research·Corinne JoffreLionel Bretillon
Apr 13, 2012·Journal of Lipid Research·Tzu Pin ShentuIrena Levitan
Apr 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Céline PrunetGérard Lizard
Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G LizardP Gambert
Jan 5, 2000·The American Journal of Clinical Nutrition·P MeeraraniB Hennig
Aug 8, 2017·Redox Biology·F LuchettiL Iuliano
Apr 13, 2000·Neurochemistry International·H KölschM L Rao

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