PMID: 6408470Feb 1, 1983Paper

Induction of sister-chromatid exchanges by DNA-damaging agents and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in synchronous Chinese hamster ovary (CHO) cells

Mutation Research
H NagasawaJ B Little

Abstract

Synchronous CHO cells were obtained by mitotic selection; synchrony was maintained up to the 5th cell cycle. The mitotic cells were seeded into T-25 flasks or P-60 plastic petri dishes, and cultured for 1 h at 37 degrees C, then the cells were treated by X-ray, UV light, and mitomycin C. The cells were then cultured for 2 cell cycles with TPA and BrdUrd and sister-chromatid exchanges (SCE) analyzed by the FPG method. Following X-irradiation, the frequency of induced SCE increased linearly with dose reaching a maximum of 19.8 times the control frequency after 200 rad. With higher doses, the SCE frequency declined. In the presence of TPA, SCE frequencies were 1.8 times control levels for all X-ray doses studied (0-800 rad), the frequency seen in non-irradiated cultures treated with TPA. The induced SCE frequency also increased linearly following treatment with UVL and mitomycin C, reaching levels higher than 1.8 times controls with doses exceeding 2.5 J/m2 UVL or mitomycin C (30 min). In the presence of TPA, the SCE frequencies increased to 1.8 times controls following low UVL and mitomycin C doses, but were not influenced by TPA in the higher dose range (above 2.5 J/m2 or 10(-10) M mitomycin C. Most of the SCE were induced by X-...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A R Kinsella, M Radman
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H Nagasawa, J B Little
Aug 1, 1972·Journal of Cellular Physiology·H Nagasawa, W C Dewey
Jan 4, 1973·Biochemical and Biophysical Research Communications·G W TeeborK J Zachary
Aug 27, 1981·Biochimica Et Biophysica Acta·M A Ritter, J R Williams
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I Emerit, P Cerutti
Aug 1, 1958·The Journal of Experimental Medicine·J H TJIO, T T PUCK

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Citations

Mar 1, 1988·Mutation Research·H NagasawaJ B Little
Jun 1, 1984·Environmental Research·F P Perera
Jun 6, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Anya EngenTakamitsu A Kato

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