Induction of SOCS-3 is insufficient to confer IRS-1 protein degradation in 3T3-L1 adipocytes

Biochemical and Biophysical Research Communications
Fang He, Jacqueline M Stephens

Abstract

Insulin receptor substrate (IRS)-1 is a key protein in insulin signaling. Several studies have shown that the expression of IRS-1 can be modulated by protein degradation via the proteasome and the degradation of IRS-1 can be related to insulin-resistant states. The degradation of IRS-1 has been shown to be induced by SOCS-1 and SOCS-3 via the ubiquitin pathway. The goal of our study was to determine if the induction of SOCS-3 correlated with increased IRS-1 degradation in cultured 3T3-L1 adipocytes. Interestingly, our studies have shown that there is little correlation between the induction in SOCS-3 expression and the degradation of IRS-1 in mature 3T3-L1 adipocytes. Our results clearly demonstrate that treatment with leukemia inhibitory factor (LIF) or cardiotrophin (CT)-1 strongly induces the expression of SOCS-3 in mature 3T3-L1 adipocytes, but does not affect the degradation of IRS-1. On the contrary, tumor necrosis factor (TNF) alpha and insulin, which very weakly induce SOCS-3 expression, have profound effects on IRS-1 degradation. In summary, our results indicate that the expression of SOCS-3 does not correlate with the degradation of IRS-1 proteins in fat cells.

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Citations

Aug 5, 2010·The Journal of Biological Chemistry·Carlos GorbeaMartin Rechsteiner
Dec 12, 2012·Veterinary Immunology and Immunopathology·Mario Delgado-OrtegaFrançois Meurens
Jan 24, 2012·Cellular Signalling·Youde JiangJena J Steinle
Apr 18, 2009·Molecular Aspects of Medicine·Tobias JungTilman Grune
Sep 21, 2006·American Journal of Physiology. Endocrinology and Metabolism·Z Elizabeth FloydJacqueline M Stephens
Feb 27, 2020·American Journal of Physiology. Endocrinology and Metabolism·Liping ZhangWilliam E Mitch

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