PMID: 9639693Jun 26, 1998Paper

Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants

Mutation Research
Tomás Gichner, M J Plewa

Abstract

The induction and measurement of DNA damage in nuclei of plant tissues is a new area of study with the alkaline single cell gel electrophoresis/comet assay. Methods to isolate plant cell nuclei cause high levels of DNA damage which are detected by the comet assay. We developed a method to isolate nuclei from leaf tissue of Nicotiana tabacum (a1+/a1; a2+/a2) in a modified Sörensen buffer that resulted in constant, low tail moment values for the negative controls. After treating intact tobacco plants with 1-8 mM ethyl methanesulfonate (EMS) we obtained a direct concentration-response with an average median tail moment of 65.9+/-4.4 micro(m) for plants exposed to the highest EMS concentration as compared to the median control tail moment value of 4.1+/-0.8. We found that the highest resolution was obtained with electrophoretic conditions of 0.74 V/cm at 300 mA for 20 min. Multiple leaves could be analyzed per plant within each treatment group and the tail moments were not significantly different. Tobacco seedlings were treated with EMS in the same manner as used for the comet assay and mutations were induced in the leaf primordia. The mean mutant frequency for the control was 1.46+/-0.20 mutant sectors/leaf. The mutant frequency i...Continue Reading

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jan 1, 1984·Environmental Mutagenesis·M J PlewaE D Wagner
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill

❮ Previous
Next ❯

Citations

Aug 11, 2005·Science in China. Series C, Life Sciences·Aijun LinYiping Tong
Apr 23, 2008·Cell Biology and Toxicology·Alok DhawanDevendra Parmar
Jun 13, 2008·Environmental Monitoring and Assessment·Rajarshi ChakrabortyAnita Mukherjee
Oct 2, 2013·Journal of Applied Genetics·Jolanta Kwasniewska, Miroslaw Kwasniewski
Dec 2, 1999·Mechanisms of Ageing and Development·G KoppenL Verschaeve
Mar 16, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S W ChiuD Moore
Oct 22, 2003·Mutation Research·Chutchalida SriussadapornDai Simazaki
May 24, 2011·International Journal of Phytoremediation·Rajarshi Chakraborty, Anita Mukherjee
Jan 30, 2014·PloS One·Jolanta Kwasniewska, Agnieszka Mikolajczyk
Apr 24, 2008·Mutation Research·Tomás GichnerJirina Száková
Sep 4, 2015·Environmental Pollution·Caroline LanierAnnabelle Deram
May 5, 2012·Environmental and Molecular Mutagenesis·Jolanta KwasniewskaBozena Kolano
Jul 27, 2005·Mutation Research·Tomás GichnerMichael J Plewa
Oct 20, 2009·Environmental Pollution·M VillariniM Moretti
Nov 3, 2009·Environmental Pollution·Helen H TaiDavid F Karnosky
Dec 21, 2014·Mutagenesis·Bertrand PourrutFrancis Douay
Dec 21, 2014·Mutagenesis·John Einset, Andrew R Collins
Feb 18, 2006·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Zofia E GagnonSteven Hicks
Aug 31, 2002·Environmental and Molecular Mutagenesis·Michael J PlewaElizabeth D Wagner
Apr 18, 2008·Environmental and Molecular Mutagenesis·Mariyana Georgieva, Lubomir Stoilov
Aug 2, 2019·Plant Signaling & Behavior·Elif ÇakmakYıldız Aydın
Mar 11, 2000·Environmental and Molecular Mutagenesis·M MenkeI Schubert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.