PMID: 9541645Apr 29, 1998Paper

Induction of somatic intrachromosomal recombination inversion events by cyclophosphamide in a transgenic mouse model

Mutation Research
P J SykesA A Morley

Abstract

Somatic intrachromosomal recombination (SICR) can result in chromosomal inversion and deletion, mechanisms which are important in carcinogenesis. We have utilised a transgenic mouse model to study SICR inversion events in spleen cells. The transgenic construct is designed so that expression of an Escherichia coli lacZ transgene only occurs in a cell when an SICR inversion event occurs in the region of the transgene. The inversion events can then be detected by histochemical staining of frozen spleen sections for transgene expression and by polymerase chain reaction across the inversion breakpoints. The spontaneous inversion frequency in spleen rose 2-fold from 1.54 +/- 0.24 x 10(-4) (mean +/- SE) in 4-month-old transgenic mice to 3.12 +/- 0.67 x 10(-4) in 22-month-old mice. Four- or 8-month-old mice were treated with a single intraperitoneal injection of cyclophosphamide, with doses ranging from 0.01 to 100 mg/kg. The animals were killed 3 days after treatment. A significant induction of SICR inversions was detected at all doses with a 3.2-fold maximum induction of inversions detected at 10 mg/kg. These results suggest that the transgenic mouse model used here may be a sensitive model for studying the role of SICR in mutation a...Continue Reading

References

Sep 21, 1977·Archives of Toxicology·R Fahrig
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Apr 1, 1992·Mutation Research·S A GristA A Morley
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S W KohlerJ M Short
Jan 1, 1991·Environmental and Molecular Mutagenesis·B C Myhr
Jan 1, 1991·Environmental and Molecular Mutagenesis·S W KohlerJ M Short
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C D JamesW K Cavenee
Dec 1, 1989·Mutation Research·R H SchiestlP J Hastings
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D R TurnerA A Morley
Jan 1, 1986·Environmental Mutagenesis·J L Dempsey, A A Morley
Sep 1, 1984·Mechanisms of Ageing and Development·K J TrainorA A Morley
May 1, 1982·Mechanisms of Ageing and Development·A A MorleyR Holliday
Jun 1, 1994·Mutation Research·J R MurtiJ C Schimenti
Jan 1, 1993·Mutation Research·J L DempseyA A Morley
May 1, 1993·Genes, Chromosomes & Cancer·Z LouK Huebner

❮ Previous
Next ❯

Citations

May 26, 2001·ANZ Journal of Surgery·R CooterG Maddern
Jul 11, 2006·Annual Review of Genomics and Human Genetics·Louise van der Weyden, Allan Bradley
Sep 28, 2004·Radiation Research·Antony M HookerPamela J Sykes
May 26, 2016·Radiation Research·Rebecca J OrmsbyPamela J Sykes
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·P J SykesA M Hooker
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Pamela J SykesMadhava Bhat
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Pamela J Sykes, Tanya K Day
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Tanya K DayPamela J Sykes
Dec 5, 2002·Physiological Genomics·Louise van der WeydenAllan Bradley
Jul 7, 2007·International Journal of Radiation Biology·Tanya K DayPamela J Sykes
Jan 26, 2010·Radiation Research·Benjamin J BlythPamela J Sykes
Sep 1, 2004·DNA Repair·Carrie A Hendricks, Bevin P Engelward

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.