Induction of Sp1 activity by prolactin and interleukin-2 in Nb2 T-cells: differential association of Sp1-DNA complexes with Stats

Molecular and Cellular Endocrinology
C K Too

Abstract

The induction of the transcription factor Sp1 by prolactin (PRL) and interleukin-2 (IL-2) was investigated in the PRL- and IL-2 responsive rat Nb2 T-cell line. Western analysis showed a rapid increase in Sp1 synthesis in Nb2 cells in response to PRL or IL-2. Elevation of Sp1 protein levels occurred within 15 min following PRL or IL-2 stimulation, reached a maximum by 1 h and was inhibited by cycloheximide, indicating de novo protein synthesis. Interestingly, dilution of confluent, growth-arrested Nb2 cells to low density also caused a rapid elevation in Sp1 suggesting that growth arrest may down-regulate Sp1 synthesis. Electrophoretic mobility shift assays using an Sp1 consensus oligonucleotide as probe showed a rapid but transient formation of a single PRL-inducible complex at 30 min. In contrast, three IL-2-inducible complexes were formed at 30 min and persisted to at least 60 min. Mobility shift interference assays using specific Stat antibodies failed to detect Stat1alpha, Stat3 or Stat5 in the 30 min PRL-inducible complex. In contrast, the IL-2 induced complexes contained Stat3 alone at 30 min and both Stat3 and Stat5 at 60 min. The PRL- and IL-2-inducible complexes did not contain the tumor suppressor protein, p53. The ti...Continue Reading

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Citations

Mar 2, 2005·Molecular and Cellular Endocrinology·Anita S GoldharRussell C Hovey
Nov 6, 2001·Molecular and Cellular Endocrinology·M Book McAlexander, L Y Yu-Lee
May 21, 1998·Antisense & Nucleic Acid Drug Development·C K Too
Jun 26, 2001·Journal of Cellular Physiology·A R BlackJ Azizkhan-Clifford
Jul 20, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·T MalewskiL Zwierzchowski
Aug 21, 2007·European Journal of Immunology·Jianhua YuMichael A Caligiuri
May 28, 2005·The Biochemical Journal·Padraic G P O'MalleyCatherine K L Too
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A MartinoB H Nelson

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