PMID: 3756217Oct 1, 1986Paper

Induction of spermidine/spermine N1-acetyltransferase in needle-punctured rat lens as a model of traumatic cataract

Biochimica Et Biophysica Acta
S MaekawaM Yokoyama

Abstract

Isolated rat lens was punctured with a needle at a single point in the equatorial region and was incubated at 37 degrees C. Spermidine/spermine N1-acetyltransferase activity was increased about 5-fold at 8 h after the puncture. Concomitantly, putrescine content in the lens increased markedly at 8-16 h after the puncture, while spermidine levels were slightly depressed. Pretreatment of the lens with actinomycin D or cycloheximide blocked the increases of spermidine/spermine N1-acetyltransferase activity and putrescine content. Ornithine decarboxylase, on the other hand, was not induced to a detectable degree by this stimulus and 5 mM difluoromethylornithine could not block the increase of putrescine content. Polyamine oxidase showed a relatively constant activity that was sufficient for the metabolism of newly formed N1-acetylspermidine. These results suggested that, in the punctured lens, the polyamine levels were regulated predominantly by the activity of spermidine/spermine N1-acetyltransferase, but not by the induction of ornithine decarboxylase.

References

Jan 1, 1979·Perspectives in Biology and Medicine·H G Williams-Ashman, Z N Canellakis
Sep 14, 1971·Biochimica Et Biophysica Acta·E Cotlier
Jul 17, 1981·Biochimica Et Biophysica Acta·I Matsui, A E Pegg
Oct 12, 1981·Biochimica Et Biophysica Acta·O SuzukiY Katsumata
Jan 1, 1981·The International Journal of Biochemistry·F N Bolkenius, N Seiler
Feb 1, 1981·Experimental Eye Research·S Uga
Dec 1, 1980·Biochimica Et Biophysica Acta·N SeilerB Knödgen

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Citations

Jan 1, 1988·The International Journal of Biochemistry·T TsukadaK Nakashima
May 1, 1988·Experimental Eye Research·S MaekawaK Nakashima
Dec 8, 1989·Biochimica Et Biophysica Acta·S MaekawaK Nakashima
Jan 1, 1991·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·N OhashiK Nakashima

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