PMID: 6537985Feb 1, 1984Paper

Induction of temperature-sensitive 6-thioguanine-resistant mutants as a measure of base-pair substitution mutations in Chinese hamster ovary cells

Mutation Research
J P O'Neill

Abstract

A method which allows quantification of the frequency of temperature-sensitive (ts) 6-thioguanine-resistant mutants of Chinese hamster ovary cells is described. These mutants, as well as non-ts type of mutant, contain altered hypoxanthine-guanine phosphoribosyl transferase (HGPRT) enzyme activity. The frequency of these altered enzyme mutants allows estimation of the fraction of total mutagenic events in the hgprt gene which results from base-pair substitution and thus provides a measure of the type of lesions induced by mutagenic agents. With an alkylating agent, ethyl methanesulfonate, 16-22% of the total induced mutants show these altered protein phenotypes, while none were found with the putative frameshift mutagen, ICR-191.

References

Jan 1, 1979·Cell·C T Caskey, G D Kruh
Jan 1, 1976·Annual Review of Biochemistry·J W Drake, R H Baltz

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