PMID: 6972536Apr 1, 1981Paper

Induction of terminal deoxynucleotidyl transferase and Lyt antigens with thymosin: identification of multiple subsets of prothymocytes in mouse bone marrow and spleen

Proceedings of the National Academy of Sciences of the United States of America
I GoldschneiderA L Goldstein

Abstract

Thymosin (fraction 5 and synthetic alpha 1 peptide) induced prothymocytes in mouse bone marrow and spleen to express terminal deoxynucleotidyl transferase (TdT; DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase, EC 2.7.7.31) or Lyt-1+, 2+, 3+ alloantigens (or both) after brief incubation in vitro. Three antigenic phenotypes were generated: (i) TdT+ Lyt+, (ii) TdT- Lyt+, and (iii) TdT+ Lyt-. The TdT+ Lyt+ phenotype was expressed by 80% of prothymocytes in bone marrow and 30% of prothymocytes in spleen from normal mice. The TdT- Lyt+ phenotype was expressed by 81% of prothymocytes in bone marrow from athymic mice. More than 80% of TdT+ bone marrow cells from normal and athymic mice expressed Lyt antigens after thymosin treatment. We interpret these observations as suggesting that (i) most TdT+ hemopoietic cells in normal and athymic mice are thymocyte progenitors; (ii) two independent lineages of prothymocytes exist, one that expresses TdT and another that does not, (iii) commitment of prothymocytes to the TdT+ cell pathway is partially regulated by a thymic feedback mechanism; and (iv) the bone marrow preferentially produces TdT+ prothymocytes, whereas the spleen may serve as a repository f...Continue Reading

References

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Citations

Jan 1, 1983·Cancer Immunology, Immunotherapy : CII·H IshitsukaY Yagi
Mar 15, 1990·Mechanisms of Ageing and Development·A SharpA Globerson
Jun 1, 1983·Immunopharmacology·M M Zatz, A L Goldstein
Jan 1, 1982·International Journal of Immunopharmacology·E DonskayaR Simon
Oct 1, 1988·Immunology and Cell Biology·K F BradstockC Luxford
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A A HaritosB L Horecker
Feb 1, 1987·The Journal of Experimental Medicine·Y H Park, D G Osmond
Jan 1, 1987·Immunologic Research·J J van Dongen, W M Comans-Bitter
Aug 2, 1985·Journal of Immunological Methods·A A Haritos, B L Horecker
Jul 1, 1992·Archives of Biochemistry and Biophysics·S FrillingosO Tsolas
Mar 1, 1984·Cancer Treatment Reviews·R V SmalleyG B Thurman
Jan 1, 1982·Leukemia Research·Y MaruyamaJ M Feola
Apr 1, 1986·Acta Neurologica Scandinavica·B KällénH Klementsson
May 1, 1982·Cellular Immunology·I GoldschneiderF J Bollum
Feb 1, 1985·Cellular Immunology·K F BradstockF J Bollum
Mar 1, 1981·Journal of Clinical Pathology·A V Hoffbrand, G Janossy
Mar 1, 1987·International Reviews of Immunology·D G Osmond, Y H Park
Nov 1, 1983·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R DaculsiJ F Duplan

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