Induction of Th2 cytokine expression for p27-specific IgA B cell responses after targeted lymph node immunization with simian immunodeficiency virus antigens in rhesus macaques

The Journal of Infectious Diseases
S KawabataH Kiyono

Abstract

To determine if there is an association between the isotype of simian immunodeficiency virus (SIV)-specific B cell responses and the profile of Th1 and Th2 cytokine expression, rhesus macaques were immunized with SIV antigens via the iliac lymph nodes, using a targeted lymph node (TLN) immunization procedure. When CD4+ T cells purified from antigen-stimulated peripheral blood mononuclear cells were analyzed, the levels of Th2 cytokine production were gradually increased after the second and third immunizations. However, interferon-gamma production did not change. Analysis of SIV-specific B cell responses revealed that the main isotype was IgG after the second and third immunizations. In addition, a peak of SIV-specific IgA B cell responses was noted following the third immunization. These findings suggest that the induction of Th2 type responses in TLN-immunized rhesus macaques reflects the sequence of initial induction of SIV-specific IgG-producing cells followed by IgA-secreting cells.

Citations

Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joseph M ThompsonRobert E Johnston
Mar 18, 2015·The World Allergy Organization Journal·Gabriela Senti, Thomas M Kündig
Oct 26, 2012·Human Vaccines & Immunotherapeutics·Pål JohansenGabriela Senti
Oct 7, 2011·Virology Journal·Ziyaad Valley-OmarEdward P Rybicki
Jan 18, 2005·Cytokine·Stefan OrangeAnnemarie Hennessy
Aug 15, 2009·Current Opinion in Allergy and Clinical Immunology·Gabriela SentiThomas M Kündig
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joseph M ThompsonRobert E Johnston

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