Induction of the apolipoprotein AI promoter by Sp1 is repressed by saturated fatty acids

Metabolism: Clinical and Experimental
Michael J HaasArshag D Mooradian

Abstract

Insulin induces transcription of the hepatic apolipoprotein AI (apo AI) gene by increasing Sp1 binding to the promoter. To determine the effect of fatty acids on this process, HepG2 cells cotransfected with the plasmid pAI.474.CAT containing the full-length apo AI promoter and the Sp1-expressing plasmid, pCMV-Sp1, were studied. Chloramphenicol acetyl transferase (CAT) activity (% acetylation) increased 1.98-fold in cells receiving the Sp1 expression construct relative to control cells (46.4% +/- 0.6% v 23.4% +/- 1.3%, P < .05). Treatment of cells with 3 saturated fatty acids, stearic, myristic, and palmitic acid, repressed the ability of exogenous Sp1 to induce apo AI reporter gene expression (15.2% +/- 1.7%, 22.5% +/- 0.3%, 22.9% +/- 0.1%, 23.5% +/- 0.8%, respectively, P < .05). Unsaturated fatty acids, oleic, linoleic, or linolenic acid had no effect on Sp1-mediated induction of the apo AI promoter. In the presence of the trans fatty acids, CAT activity in the Sp1-transfected cells was similar to control cells (16.7% +/- 3.3%, 19.3% +/- 0.5%, and 21.0% +/- 2.1% acetylation in cells exposed to elaidic acid, linolelaidic, or linolenelaidic acid, respectively). In cells treated with an equimolar mixture of oleic acid and stearic...Continue Reading

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Citations

Mar 8, 2014·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Arshag D Mooradian, Michael J Haas
Oct 30, 2007·The Medical Clinics of North America·Danielle Lann, Derek LeRoith
Aug 21, 2007·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Stefan P J DullensRonald P Mensink
Jan 8, 2013·Molecular Nutrition & Food Research·Carlota OleagaVéronique Noé
Mar 19, 2014·Pharmaceutical Biology·Michael J HaasArshag D Mooradian
Apr 5, 2008·Obesity·Arshag D MooradianNorman C W Wong
Jul 18, 2020·Clinical Science·Lei ZhangXiao-Jing Zhang
Feb 6, 2020·Drugs·Julien J Feghaly, Arshag D Mooradian
Oct 25, 2017·Cell Biology International·Jun-Fa ZengZuo Wang

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