Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT

Molecular Microbiology
J StülkeG Rapoport

Abstract

Glucose is the preferred carbon and energy source of Bacillus subtilis. It is transported into the cell by the glucose-specific phosphoenolpyruvate:sugar phosphotransferase system (PTS) encoded by the ptsGHI locus. We show here that these three genes (ptsG, ptsH, and ptsI) form an operon, the expression of which is inducible by glucose. In addition, ptsH and ptsl form a constitutive ptsHI operon. The promoter of the ptsGHI operon was mapped and expression from this promoter was found to be constitutive. Deletion mapping of the promoter region revealed the presence of a transcriptional terminator as a regulatory element between the promoter and coding region of the ptsG gene. Mutations within the ptsG gene were characterized and their consequences on the expression of ptsG studied. The results suggest that expression of the ptsGHI operon is subject to negative autoregulation by the glucose permease, which is the ptsG gene product. A regulatory gene located upstream of the ptsGHI operon, termed glcT, was also identified. The GlcT protein is a novel member of the BglG family of transcriptional antiterminators and is essential for the expression of the ptsGHI operon. A deletion of the terminator alleviates the need for GlcT. The ac...Continue Reading

Citations

Nov 11, 2006·Applied Microbiology and Biotechnology·Martin Tangney, Wilfrid J Mitchell
Mar 3, 2004·Research in Microbiology·Marie-Françoise HulloIsabelle Martin-Verstraete
Nov 20, 2002·Research in Microbiology·David B GreenbergMilton H Saier
May 7, 1998·Molecular Microbiology·J ReizerW Hillen
Jan 11, 2013·Nucleic Acids Research·Caroline ClerteEmmanuel Margeat
Oct 14, 2005·Nucleic Acids Research·Ralph BertramWolfgang Hillen
Nov 17, 2010·Antimicrobial Agents and Chemotherapy·Anna StarońThorsten Mascher
Sep 12, 2012·Antimicrobial Agents and Chemotherapy·Diana WolfThorsten Mascher
Oct 27, 2001·Applied and Environmental Microbiology·J Lee, H P Blaschek
May 19, 2006·Journal of Bacteriology·Stefan SchönertMichael K Dahl
Jul 20, 2010·Journal of Bacteriology·Diana WolfThorsten Mascher
Jun 23, 2009·Journal of Bacteriology·Heike PreisSabine Brantl
Oct 24, 2006·Journal of Bacteriology·Marie-Françoise HulloIsabelle Martin-Verstraete
Feb 13, 2007·Journal of Bacteriology·Verena Engels, Volker F Wendisch
Aug 23, 2011·Journal of Bacteriology·Letal I SalzbergJohn D Helmann
Aug 10, 2002·Journal of Bacteriology·Isabelle GuillouardIsabelle Martin-Verstraete
Aug 24, 2002·Journal of Bacteriology·Sandrine AugerIsabelle Martin-Verstraete
Nov 18, 2003·Journal of Bacteriology·Harald NothaftFritz Titgemeyer

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