Induction of the cellular microRNA, Hs_154, by West Nile virus contributes to virus-mediated apoptosis through repression of antiapoptotic factors.

Journal of Virology
Jessica L SmithAlec J Hirsch

Abstract

MicroRNAs (miRNAs) are a class of noncoding small RNAs that regulate multiple cellular processes, as well as the replication and pathogenesis of many DNA viruses and some RNA viruses. Examination of cellular miRNA profiles in West Nile virus (WNV)-infected HEK293 and SK-N-MC cells revealed increased expression of multiple miRNA species. One of these miRNAs, Hs_154, was significantly induced not only in WNV-infected neuronal cells in culture but also in the central nervous system tissues of infected mice and, upon transfection, caused a significant reduction in viral replication. Analysis of mRNA transcripts enriched through immunoprecipitation of the RNA-induced silencing complex identified several transcripts that contain seed sequence matches to Hs_154 in their 3' untranslated regions (UTRs). Two of these targets, the CCCTC-binding factor (CTCF) and the epidermal growth factor receptor (EGFR)-coamplified and overexpressed protein (ECOP/VOPP1) proteins display reduced expression in WNV-infected cells, and the 3' UTRs of these transcripts were sufficient to cause downregulation of expression in infected cells or in cells transfected with Hs_154, findings consistent with miRNA targeting of these transcripts. CTCF and ECOP have b...Continue Reading

References

Mar 6, 1992·Cell·P MombaertsV E Papaioannou
Jul 1, 1982·The American Journal of Tropical Medicine and Hygiene·E A HenchalW E Brandt
Aug 6, 2002·Annals of Internal Medicine·Lyle R Petersen, Anthony A Marfin
Dec 25, 2002·Emerging Infectious Diseases·Joo-Sung YangDavid B Weiner
Oct 22, 2003·Cell·Dianne S SchwarzPhillip D Zamore
Oct 22, 2004·Emerging Infectious Diseases·Anne Labowitz KleeDenis Nash
Nov 20, 2004·Science·Yukihide TomariPhillip D Zamore
Apr 23, 2005·Science·Charles-Henri LecellierOlivier Voinnet
Jul 19, 2005·Cancer Research·Jennifer A ChanKenneth S Kosik
Sep 6, 2005·Science·Catherine L JoplingPeter Sarnow
Sep 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Amelia CimminoCarlo M Croce
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Aug 31, 2006·Methods in Enzymology·Alexander I SaeedJohn Quackenbush
Oct 31, 2006·Nature Genetics·Eugene BerezikovRonald H A Plasterk
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·I Behm-AnsmantE Izaurralde
Jun 2, 2007·Molecular Cell·Tsung-Cheng ChangJoshua T Mendell
Jun 23, 2007·Experimental Cell Research·Tie Li, Luo Lu
Oct 11, 2007·PloS One·Haoquan WuN Manjunath
Oct 19, 2007·Nature·Irene M PedersenMichael David
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fedor V KarginovGregory J Hannon
Oct 8, 2008·The Journal of Experimental Medicine·Elizabeth Yee-Wai ChoyDong-Yan Jin
Jan 16, 2009·Journal of Hepatology·Yoshiki MurakamiKunitada Shimotohno
Apr 17, 2009·Journal of Virology·Guruprasad R MedigeshiJay A Nelson
May 20, 2009·Genes & Development·Jennifer L Umbach, Bryan R Cullen
Apr 27, 2010·Current Drug Targets·Virginija Jazbutyte, Thomas Thum
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Diwakar SanthakumarAmy H Buck
Aug 24, 2010·Antiviral Research·Guang-ling ZhangHua Tang

❮ Previous
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Citations

Jan 20, 2016·Expert Reviews in Molecular Medicine·Mahmoud IranpourSaeid Ghavami
Aug 8, 2014·The Biochemical Journal·Katell Bidet, Mariano A Garcia-Blanco
Apr 8, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yuanpeng ZhangPuyan Chen
May 29, 2016·Virology Journal·Lorena BaviaClaudia Nunes Duarte Dos Santos
Aug 29, 2017·Viruses·Toru OkamotoYoshiharu Matsuura
Nov 1, 2017·Scientific Reports·Jasmine SamalPerumal Vivekanandan
Jun 8, 2018·Viral Immunology·Rafaella Nascimento FerreiraAna Cecília Ribeiro Cruz
May 31, 2018·Archives of Microbiology·Yao-Zhong DingYong-Guang Zhang
Aug 10, 2012·Journal of Virology·Nicholas L BairdJack H Nunberg
Sep 21, 2013·Physiological Genomics·Gokul SwaminathanSonia Navas-Martin
Nov 7, 2019·Pathogens·Bi-Hung Peng, Tian Wang
Sep 19, 2019·Médecine sciences : M/S·Paula LópezSébastien Pfeffer
Mar 3, 2020·Frontiers in Microbiology·Ashleigh R Murphy SchaferAlec J Hirsch
Apr 22, 2018·Biochemical Society Transactions·Elisa VicenziEdwin A Yates
Feb 20, 2019·Viruses·Francine AzouzMukesh Kumar
Oct 20, 2018·Frontiers in Genetics·Erika GirardiSébastien Pfeffer
Apr 13, 2021·Frontiers in Microbiology·Yuhong PanRenyong Jia
Apr 27, 2021·Molecular Therapy. Nucleic Acids·Javid Sadri NahandHamed Mirzaei

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