Induction of the d-Amino Acid Oxidase from Trigonopsis variabilis.

Applied and Environmental Microbiology
R HornerL Fischer

Abstract

Induction of the d-amino acid oxidase (EC. 1.4.3.3) from the yeast Trigonopsis variabilis was investigated by using a minimal medium containing glucose as the carbon and energy source, (NH(inf4))(inf2)SO(inf4) as the nitrogen source, and various d- and dl-amino acid derivatives as inducers. The best new inducers found were N-carbamoyl-d-alanine, N-acetyl-d-tryptophan, and N-chloroacetyl-d-(alpha)-aminobutyric acid; when the induction effects of these compounds were compared with the effects of d-alanine as the nitrogen source and inducer, the resulting activities of d-amino acid oxidase per gram of dried yeast were 4.2, 2.1, and 1.5 times higher, respectively. The optimum concentration of the best inducer, N-carbamoyl-d-alanine, was 5 mM. This inducer could also be used in its racemic form. The induction was pH dependent. After cultivation of the yeast in a 50-liter bioreactor, d-amino acid oxidase activity of about 3,850 (mu)kat (231,000 U) was obtained. In addition, production of the d-amino acid oxidase was found to be significantly dependent on the metal salt composition of the medium. Addition of zinc ions was required to obtain high d-amino acid oxidase levels in the cells. The optimum concentration of ZnSO(inf4) was abou...Continue Reading

References

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Citations

Feb 7, 2007·Applied Microbiology and Biotechnology·Birgit GeuekeWerner Hummel
Nov 10, 2009·Canadian Journal of Microbiology·Susmita SinghR L Bezbaruah
Mar 10, 2009·Biochemistry. Biokhimii︠a︡·A Gholizadeh, B B Kohnehrouz
Oct 12, 1996·Annals of the New York Academy of Sciences·L FischerF Wagner
Dec 18, 2007·Applied Microbiology and Biotechnology·Loredano PollegioniMirella S Pilone
Sep 10, 2003·Yeast·Gianluca MollaLoredano Pollegioni
Jul 31, 1999·Applied and Environmental Microbiology·M Gabler, L Fischer
Oct 29, 1998·Protein Expression and Purification·G MollaL Pollegioni
Oct 12, 2000·Enzyme and Microbial Technology·M GablerL Fischer

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