PMID: 2105080Jan 1, 1990Paper

Induction of the enzyme aldose reductase in a lens epithelial cell line from a transgenic mouse

Archives of Biochemistry and Biophysics
P RussellL O Merola

Abstract

A lens epithelial cell line established from a transgenic mouse synthesizes high levels of the enzyme aldose reductase which converts sugars to polyols. This enzyme has been implicated in the formation of sugar cataracts in animals and with diabetic complications in man. The mouse aldose reductase has been characterized and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis has an apparent molecular mass of 38,000, similar to the enzyme in rat and man. The cellular enzyme is inhibited by two aldose reductase inhibitors: Sorbinil (IC50 = 1.8 X 10(-7) M) and Alcon 1576 (IC50 = 7.8 X 10(-8) M). The amount and the specific activity of the aldose reductase can be further increased in the cells by raising the osmolarity of the medium to 500 mOSM. Although the amount of aldose reductase is increased approximately sevenfold under these conditions, alpha-crystallin, one of the main lens specific proteins, remained at about the same concentration. No detectable increase in sorbitol was found within the cells, in contrast to published reports on renal cells in which this polyol increases under similar hyperosmotic conditions; however, in the lens cells there was a five-fold increase in the inositol content, suggesting that this ...Continue Reading

References

Mar 27, 1987·Science·K A MahonH Westphal
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S M BagnascoM B Burg
Jun 1, 1988·The American Journal of Physiology·S M BagnascoM B Burg
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W HendriksW W de Jong
Feb 1, 1983·Biochemical Medicine·H B MarkusH Harris
Aug 1, 1981·Experimental Eye Research·T S Kern, R L Engerman
Jun 1, 1981·Analytical Biochemistry·P F KadorJ H Kinoshita
Oct 1, 1982·Experimental Eye Research·P RussellJ H Kinoshita
Jan 1, 1981·Differentiation; Research in Biological Diversity·J Piatigorsky

❮ Previous
Next ❯

Citations

Nov 1, 1991·Pflügers Archiv : European journal of physiology·M A WatskyJ L Rae
Jan 11, 1991·Free Radical Biology & Medicine·P RussellJ S Zigler
Jan 19, 2012·Experimental Diabetes Research·Barbara LewkoApolonia Rybczyńska
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·A Bhatnagar, S K Srivastava
Jun 1, 1997·Glia·C Jacquin-Becker, G Labourdette
Mar 10, 1999·The American Journal of Physiology·J FischbargJ P Koniarek
Apr 1, 1997·Current Eye Research·F P Diecke, A Beyer-Mears
Apr 13, 1999·The American Journal of Physiology·A RichJ L Rae

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
P F KadorK Sugiyama
The Consultant Pharmacist : the Journal of the American Society of Consultant Pharmacists
Rebecca B Sleeper
© 2021 Meta ULC. All rights reserved