PMID: 7520995Sep 1, 1994Paper

Induction of the genes RAD54 and RNR2 by various DNA damaging agents in Saccharomyces cerevisiae

Mutation Research
Dietrich Averbeck, S Averbeck

Abstract

The relationship between the induction of the genes RAD54 and RNR2 and the induction and repair of specific DNA lesions was studied in the yeast Saccharomyces cerevisiae using Rad54-lacZ and RNR2-lacZ fusion strains. Gene induction was followed by measuring beta-galactosidase activity. At comparable levels of furocoumarin-DNA photoadducts, RAD54 was more effectively induced by bifunctional than by monofunctional furocoumarins indicating that mixtures of monoadducts (MA) and interstrand cross-links (CL) provide a stronger inducing signal than MA. RNR2 induction kinetics were measured in relation to cell growth and survival responses after treatment with the furocoumarins 8-methoxypsoralen (8-MOP), 5-methoxypsoralen (5-MOP), 3-carbethoxypsoralen (3-CPs), 7-methyl-pyrido[3,4-c]psoralen (MePyPs) and 4,4',6-trimethylangelicin (TMA), benzo[a]pyrene (B(a)P and 1,6-dioxapyrene (1,6-DP) plus UVA, 254 nm UV radiation and cobalt-60 gamma-radiation. Induction of RNR2 took place during the DNA repair period before resumption of cell growth and clearly increased with increasing equitoxic dose levels. Treatments with furocoumarin plus 365 nm radiation (UVA) and 254 nm (UV) radiation were effective inducers whereas gene induction was relativel...Continue Reading

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Citations

May 29, 2004·Mutation Research·Andrej Dudás, Miroslav Chovanec
May 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·J X Chen, J Kagan
Feb 1, 2005·Nucleic Acids Research·Martin ToussaintRaymund J Wellinger
Dec 20, 2002·Nucleic Acids Research·Markus KiechleAnna A Friedl
Aug 14, 2001·Infection and Immunity·D C HassaneC L Pickett
Jul 5, 2007·FEMS Yeast Research·Michèle DardalhonDietrich Averbeck
Jan 23, 2013·Journal of Applied Toxicology : JAT·Giel HendriksHarry Vrieling
Dec 6, 2000·Alternatives to Laboratory Animals : ATLA·H SpielmannUNKNOWN European Centre for Validation of Alternative Methods
May 5, 2009·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·E BenfenatiC Yang
Nov 13, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BakkaliM Idaomar

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