Induction of the hsp70 Gene Promoter by Various Anticancer Drugs

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S At-AssaC Lambre

Abstract

HeLa cells containing the chloramphenicol acetyl transferase (CAT) gene under the control of the hsp70 promoter have been exposed in vitro to various anticancer drugs. Cisplatin induced CAT production with a dose-effect relationship at a non-cytotoxic dose, whereas no induction was detected with carboplatin. Etoposid induced a significant response at a cytotoxic concentration. The limited positive response with doxorubicin, daunomycin and mitoxantrone was not statistically significant. These chemicals are known to produce reactive oxygen species and induce apoptosis. No induction of the hsp70 promoter could be detected with the other cytostatic compounds that have been tested such as base analogues (5-fluorouracil, cytosine arabinoside 3'-MP), inhibitors of DNA synthesis (amethopterin, aminopterin), antimitotics (vinblastine, colchicine), and alkylating (streptozotocine, carboplatin, melphalan) or intercalating agents (bleomycin). In addition, the role of the transcription inhibitory activity of doxorubicin in this model is evidenced and the consequent question of the suitability of the reporter gene system is discussed. Our results suggest that specific genotoxic compounds are not able to induce the hsp70 promoter, and are in ...Continue Reading

References

Feb 28, 1991·The New England Journal of Medicine·J M Astrachan
Aug 1, 1989·American Journal of Respiratory Cell and Molecular Biology·P L MoseleyJ York
Oct 6, 1993·Journal of the National Cancer Institute·D R CioccaS A Fuqua
Sep 1, 1996·Toxicology and Applied Pharmacology·R AlbersR Pieters
Oct 1, 1996·Biochemistry and Molecular Biology International·J Meléndez-ZajglaV Maldonado
Jul 4, 1998·International Journal of Radiation Oncology, Biology, Physics·H MatsumotoE Kano
Jan 1, 1998·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· R P Bos B F J Weissenberger R B M Anzion

❮ Previous
Next ❯

Citations

Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C UraniM Camatini
Jan 18, 2003·Biological Chemistry·Mathias GehrmannGabriele Multhoff
Apr 12, 2015·Environmental Toxicology and Pharmacology·Lili XinSifan Guo
Jul 16, 2008·Chemistry : a European Journal·Hans Wolf SünnemannArmin de Meijere

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis