PMID: 8449875Mar 1, 1993Paper

Induction of the lambda receptor is essential for effective uptake of trehalose in Escherichia coli

Journal of Bacteriology
W Klein, Winfried Boos

Abstract

Trehalose transport in Escherichia coli after growth at low osmolarity is mediated by enzyme IITre of the phosphotransferase system (W. Boos, U. Ehmann, H. Forkl, W. Klein, M. Rimmele, and P. Postma, J. Bacteriol. 172:3450-3461, 1990). The apparent Km (16 microM) of trehalose uptake is low. Since trehalose is a good source of carbon and the apparent affinity of the uptake system is high, it was surprising that the disaccharide trehalose [O-alpha-D-glucosyl(1-1)-alpha-D-glucoside] has no problems diffusing through the outer membrane at high enough rates to allow full growth, particularly at low substrate concentrations. Here we show that induction of the maltose regulon is required for efficient utilization of trehalose. malT mutants that lack expression of all maltose genes, as well as lamB mutants that lack only the lambda receptor (maltoporin), still grow on trehalose at the usual high (10 mM) trehalose concentrations in agar plates, but they exhibit the half-maximal rate of trehalose uptake at concentrations that are 50-fold higher than in the wild-type (malT+) strain. The maltose system is induced by trehalose to about 30% of the fully induced level reached when grown in the presence of maltose in a malT+ strain or when gro...Continue Reading

References

Oct 1, 1976·European Journal of Biochemistry·T N PalmerW J Whelan
Apr 1, 1991·Molecular Microbiology·K SchmidF Titgemeyer
Nov 1, 1990·Research in Microbiology·E FrancozM Hofnung
Jun 1, 1990·Journal of Bacteriology·W BoosP Postma
Mar 1, 1986·Journal of Bacteriology·R BenzG H Vos-Scheperkeuter
Jun 1, 1986·Journal of Bacteriology·B BukauW Boos
Aug 1, 1987·Journal of Bacteriology·M Ehrmann, W Boos
Jul 1, 1987·Journal of Bacteriology·O Raibaud, E Richet
Aug 15, 1974·European Journal of Biochemistry·O Kellermann, S Szmelcman
Sep 1, 1967·European Journal of Biochemistry·M Schwartz, M Hofnung
Aug 1, 1980·Journal of Bacteriology·B R BochnerB N Ames
Jul 1, 1984·Journal of Bacteriology·J M BrassT J Larson

❮ Previous
Next ❯

Citations

Jun 19, 2008·Ecology Letters·Christine M Jessup, Brendan J M Bohannan
Feb 1, 1995·Evolution; International Journal of Organic Evolution·Michael TravisanoRichard E Lenski
Nov 1, 1993·Molecular Microbiology·H Lång, E T Palva
Feb 1, 1999·Evolution; International Journal of Organic Evolution·Brendan J M BohannanRichard E Lenski
Oct 24, 2019·Environmental Microbiology Reports·Buddhie S NanayakkaraDavid M Gordon
May 19, 2000·The Journal of Biological Chemistry·K UhlandM Ehrmann
May 15, 2001·The Journal of Biological Chemistry·E W PirokN B Schwartz
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·W Boos, H Shuman
Feb 1, 1997·Journal of Bacteriology·D MeyerW Boos
Nov 1, 2005·EcoSal Plus·Christoph Mayer, Winfried Boos
Jun 1, 1995·Journal of Bacteriology·J L Wylie, E A Worobec

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.