Abstract
SqCC/Y1, a human malignant squamous cell carcinoma, spontaneously differentiates when grown to confluence in delipidized serum-containing medium, as measured by the capacity to form detergent-insoluble cornified cell envelopes. Thus, 30% of SqCC/Y1 cells spontaneously attained the differentiated state after 6 days in culture. Exposure of SqCC/Y1 cells to 30, 100, or 300 nM hydrocortisone increased the number of mature cells, producing a 25, 100, and 225%, respective, increase in the number of differentiated cells over the spontaneous rate of maturation. Retinoic acid at levels of 3-300 nM was inhibitory, causing a 24-85% decrease in the number of differentiated cells. Simultaneous treatment with hydrocortisone and retinoic acid indicated mutual antagonism of the effects of these agents on the formation of cornified envelopes. Since hydrocortisone possesses antiangiogenic (AG), mineralocorticoid (MC) and glucocorticoid (GC) activities, steroids with different degrees of GC, MC, and AG potency were examined for their capacities to induce terminal differentiation. Only steroids with GC activity, such as dexamethasone, hydrocortisone, and RU-28362, were capable of increasing the degree of SqCC/Y1 differentiation and antagonizing th...Continue Reading
References
Jun 1, 1977·Cell·R H Rice, H Green
Aug 1, 1976·In Vitro·G H RothblatB V Howard
Dec 1, 1976·Cell·T T Sun, H Green
Dec 20, 1985·Science·R CrumJ Folkman
Jul 8, 1985·FEBS Letters·R SchmidtM Bouclier
Nov 1, 1986·Experimental Cell Research·I KingA C Sartorelli
Jan 1, 1986·Advances in Enzyme Regulation·A C SartorelliM Reiss
Oct 1, 1986·Journal of Pharmaceutical Sciences·M PonecJ C Caron
May 1, 1973·Endocrinology·D FeldmanI S Edelman
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K R Yamamoto, B M Alberts
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·E V JensenE R DeSombre
Nov 15, 1983·Biochemical Pharmacology·M N ChobertR Deraedt
Nov 1, 1980·Cell·J G Rheinwald, M A Beckett
Sep 1, 1981·Cell·E Fuchs, H Green
Jan 1, 1983·Carcinogenesis·E K ParkinsonA Emmerson
Aug 19, 1983·Science·J FolkmanS Taylor
Apr 1, 1984·Cell·M Simon, H Green
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K H KimE Fuchs
Jan 1, 1984·Journal of Steroid Biochemistry·V LelièvreF Russo-Marie
Jan 1, 1984·Journal of Steroid Biochemistry·M Moguilewsky, D Philibert
Citations
Jan 1, 1990·Journal of Cellular Physiology·S M VioletteA C Sartorelli
Jul 13, 2007·Biochemical and Biophysical Research Communications·Xavier DezitterThierry Idziorek
Jun 17, 2009·Molecular and Cellular Biology·Zhenyun YangRebecca J Chan