Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: impact of vascular endothelial growth factor

Apoptosis : an International Journal on Programmed Cell Death
Manfred InfangerDaniela Grimm

Abstract

Endothelial cells play a crucial role in the pathogenesis of many diseases and are highly sensitive to low gravity conditions. Using a three-dimensional random positioning machine (clinostat) we investigated effects of simulated weightlessness on the human EA.hy926 cell line (4, 12, 24, 48 and 72 h) and addressed the impact of exposure to VEGF (10 ng/ml). Simulated microgravity resulted in an increase in extracellular matrix proteins (ECMP) and altered cytoskeletal components such as microtubules (alpha-tubulin) and intermediate filaments (cytokeratin). Within the initial 4 h, both simulated microgravity and VEGF, alone, enhanced the expression of ECMP (collagen type I, fibronectin, osteopontin, laminin) and flk-1 protein. Synergistic effects between microgravity and VEGF were not seen. After 12 h, microgravity further enhanced all proteins mentioned above. Moreover, clinorotated endothelial cells showed morphological and biochemical signs of apoptosis after 4 h, which were further increased after 72 h. VEGF significantly attenuated apoptosis as demonstrated by DAPI staining, TUNEL flow cytometry and electron microscopy. Caspase-3, Bax, Fas, and 85-kDa apoptosis-related cleavage fragments were clearly reduced by VEGF. After 72 ...Continue Reading

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Citations

Feb 3, 2011·European Journal of Applied Physiology·Chun-Yan KangChang-Ting Liu
Oct 26, 2012·Cell Biochemistry and Biophysics·Hongyan KangXiaoyan Deng
Jan 24, 2012·Chemical Research in Toxicology·Dorota NapierskaPeter H Hoet
Feb 20, 2009·Tissue Engineering. Part a·Daniela GrimmJohann Bauer
Sep 17, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yong-Chun WangXi-Qing Sun
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Claudia UlbrichDaniela Grimm
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Claudia UlbrichDaniela Grimm
Jan 11, 2013·International Journal of Molecular Sciences·Jessica PietschDaniela Grimm
Jun 3, 2014·Cell Communication and Signaling : CCS·Elisabeth WarnkeDaniela Grimm
Mar 7, 2014·Tissue Engineering. Part B, Reviews·Daniela GrimmJohann Bauer
Jun 25, 2014·Expert Review of Proteomics·Daniela GrimmJohann Bauer
Feb 19, 2011·Expert Review of Proteomics·Daniela GrimmSarah Baatout
Mar 26, 2015·Preparative Biochemistry & Biotechnology·Claudia KochJohann Bauer
Mar 7, 2007·Journal of Cellular Biochemistry·Manfred InfangerDaniela Grimm
Feb 7, 2008·Journal of Cellular Biochemistry·Claudia UlbrichDaniela Grimm
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Aug 12, 2014·BioMed Research International·Claudia UlbrichDaniela Grimm
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Oct 30, 2008·Cell Communication and Signaling : CCS·Oliver UllrichKerstin Lang
Oct 30, 2016·Basic & Clinical Pharmacology & Toxicology·Ganna AleshchevaDaniela Grimm
May 1, 2013·International Journal of Molecular Sciences·Markus WehlandDaniela Grimm
Mar 30, 2018·Stem Cells and Development·Daniela GrimmAlamelu Sundaresan
Oct 29, 2017·Journal of Cellular Biochemistry·Ludmila B BuravkovaAnatoly I Grigoriev
Oct 26, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jirka GrosseDaniela Grimm

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