Induction of transcription factor AP-2 by inflammatory cytokines in human keratinocytes

The Journal of Investigative Dermatology
N OyamaF Kaneko

Abstract

Activator protein-2 is an important transcription factor for the activation of a number of genes. Here we report the induction of activator protein-2 in response to inflammatory cytokines such as interleukin-6 in keratinocytes. Immunoblotting and semiquantitative reverse transcriptase-polymerase chain reaction assays using normal human keratinocytes revealed that interleukin-6 caused a time- and concentration-dependent induction of activator protein-2 mRNA and protein. The increase of activator protein-2 mRNA was detected at 30 min after stimulation and that of activator protein-2 protein was at 2 h. Their levels were lower than the control levels at 24 h. The interleukin-6-dependent induction of activator protein-2 mRNA was completely blocked by adding actinomycin D, whereas it was approximately 50% affected by cycloheximide. Co-incubation with neutralizing antibodies against various inflammatory cytokines resulted in inhibition of the interleukin-6-dependent activator protein-2 induction at varying degrees, indicating an involvement of various cytokines in the activator protein-2 induction. The activator protein-2 induction was observed in keratinocytes derived from lesional skins with psoriasis or squamous cell carcinoma, an...Continue Reading

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Citations

Jan 21, 2006·Respirology : Official Journal of the Asian Pacific Society of Respirology·Ping ChenXiang Fang
Jul 12, 2011·Medicina oral, patología oral y cirugía bucal·Vlaho BrailoMelita Balija
Dec 29, 2009·Molecular and Cellular Endocrinology·Stuart Handwerger
Dec 21, 2011·The American Journal of Pathology·Changguo ChenNing Guo
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Jan 7, 2015·Asia-Pacific Journal of Clinical Oncology·Niranzena Panneer Selvam, Jayachandran Sadaksharam
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Mar 25, 2004·Physiological Genomics·You-Hong ChengStuart Handwerger

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