PMID: 6106509Oct 1, 1980Paper

Induction of tyrosine aminotransferase and amino acid transport in rat hepatoma cells by insulin and the insulin-like growth factor, multiplication-stimulating activity. Mediation by insulin and multiplication-stimulating activity receptors

Biochimica Et Biophysica Acta
J H HeatonS P Nissley

Abstract

Insulin stimulates a 2-fold increase in the amount of tyrosine aminotransferase and a 5-10-fold increase in the rate of amino acid transport in dexamethasone-treated rat hepatoma cells. In order to determine whether these effects are mediated by insulin receptors or receptors for insulin-like growth factors, we have examined the binding of 125I-labeled insulin and 125I-labeled multiplication-stimulating activity, a prototype insulin-like growth factor, and compared the biological effects of these polypeptides. Insulin and multiplication-stimulating activity cause an identical increase in transaminase activity and transport velocity; half-maximal biological effects were observed at 35 ng/mg (5.5 nM) insulin and 140 ng/ml multiplication-stimulating activity. The hepatoma cells display typical insulin receptors of appropriate specificity; half-maximal displacement of tracer insulin binding occurred at 33 ng/ml unlabeled insulin, but only at 2500 ng/ml unlabeled multiplication-stimulating activity. Specific multiplication-stimulating activity receptors also were demonstrated with which insulin did not interact even at 10 micrograms/ml. Half-maximal displacement of tracer multiplication-stimulating activity occurred at 200 ng/ml unl...Continue Reading

References

Sep 1, 1975·The Journal of Clinical Endocrinology and Metabolism·J R GavinD M Neville
May 1, 1977·The Journal of Clinical Endocrinology and Metabolism·M M RechlerL Fryklund
May 15, 1978·FEBS Letters·E Rinderknecht, R E Humbel
Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·T D Gelehrter, G M Tomkins
Feb 12, 1980·Biochemical and Biophysical Research Communications·J H Heaton, T D Gelehrter
May 15, 2002·Inorganic Chemistry·Jasmine R BryantJames M Mayer

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Citations

Sep 17, 1982·Molecular and Cellular Biochemistry·D S Straus, K J Pang
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·J Lerner
Mar 1, 1984·Clinics in Endocrinology and Metabolism
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·M M RechlerR M White
Mar 25, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Belaïd SekkaliKris Vleminckx
May 24, 1983·Biochimica Et Biophysica Acta·M A ShotwellD L Oxender
Mar 1, 1984·Clinics in Endocrinology and Metabolism·S P Nissley, M M Rechler

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