PMID: 3744191May 1, 1986Paper

Induction of unscheduled DNA synthesis in rat and hamster hepatocytes by cooked food mutagens

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
A J HowesI R Rowland

Abstract

The genotoxicity of the cooked-food mutagens 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) was studied by monitoring the induction of DNA repair (unscheduled DNA synthesis; UDS) in primary cultures of rodent hepatocytes. The hepatocytes were derived from male Sprague-Dawley rats or Syrian hamsters by collagenase perfusion and the cells were cultured for 4 hr before being exposed to various concentrations of the mutagens. DNA repair was determined by measuring incorporation of [3H]thymidine into extracted DNA over 17 hr using beta-scintillation counting. Dose-related increases in UDS were clearly seen in hamster hepatocytes treated with MeIQ, IQ and the positive control 2-acetylaminofluorene (AAF), and a weak response was induced by MeIQx and Trp-P-1. In the rat hepatocytes only MeIQ and AAF gave clear positive responses. Furthermore it was noted that all the mutagens displayed a more pronounced UDS response in hamster hepatocytes than in rat cells. Studies of the activation of MeIQ by hepatocytes to a bacterial mutag...Continue Reading

References

Jan 1, 1985·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·S Grivas, K Olsson
Oct 1, 1983·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G CaderniP Dolara
Jan 1, 1984·Carcinogenesis·J S FeltonF T Hatch
Jan 1, 1983·Toxicology and Applied Pharmacology·T J GrayS D Gangolli

❮ Previous
Next ❯

Citations

Jan 1, 1995·Environmental and Molecular Mutagenesis·M A JohanssonJ S Felton
Sep 1, 1989·Mutation Research·J D TuckerJ S Felton
Mar 1, 1991·Mutation Research·H U Aeschbacher, R J Turesky
Aug 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A J HowesJ Caldwell
Oct 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·V S Chan, J Caldwell
Dec 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Johansson, M Jägerstad
Sep 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J A BeamandB G Lake
Mar 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G Hasheminejad, J Caldwell
Nov 1, 1995·Critical Reviews in Oncology/hematology·A M LynchA R Boobis
Nov 13, 2013·Environmental Toxicology and Pharmacology·Annemieke KolkmanMinne B Heringa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.