Induction of vitellogenin gene transcription in vitro by juvenile hormone in Blattella germanica

Molecular and Cellular Endocrinology
D ComasXavier Bellés

Abstract

In the cockroach Blattella germanica, the synthesis of vitellogenin is juvenile hormone III (JH III)-dependent. We have studied the effect of JH III upon vitellogenin gene expression in periovaric fat bodies incubated in vitro. Periovaric fat bodies were obtained from cardioallatectomized females. The response to JH III was measured in terms of vitellogenin and vitellogenin mRNA after 7 h of incubation. A hormonal concentration as low as 1 nM was enough to induce vitellogenin production and its release to the medium, whereas the concentration of 10 nM produced the maximal effects. Although the response of the vitellogenin gene to JH III is fast and efficient, it seems that the action is mediated by protein factors, given that cycloheximide treatment impairs the hormonal effect. The presence in the medium of brain extract (0.5 equivalents), corpora cardiaca (one pair) or hypertrehalosemic hormone (10(-7) or 10(-8) M), partially inhibited the response to JH III.

References

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Citations

Nov 6, 2003·Insect Biochemistry and Molecular Biology·J CruzX Bellés
Nov 20, 2003·Journal of Insect Physiology·David C HaywardVirginia K Walker
May 9, 2002·Molecular and Cellular Endocrinology·S ZhouV K Walker
Jan 18, 2003·Insect Biochemistry and Molecular Biology·E B DubrovskyE M Berger
Jul 28, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Françoise Goudey-PerrièreClaude Perrière
Oct 31, 2014·PLoS Genetics·Jesus LozanoXavier Belles
Jan 10, 2006·Vitamins and Hormones·Edward M Berger, Edward B Dubrovsky
Oct 21, 2016·Scientific Reports·Hemant Gujar, Subba Reddy Palli
Sep 20, 2006·Archives of Insect Biochemistry and Physiology·S Carmen Panaitof, Michelle Pellissier Scott
Sep 13, 2017·BMC Genomics·Qiang ZhangZe Zhang
Oct 24, 2017·Annual Review of Entomology·Sourav RoyAlexander S Raikhel

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