Induction of zinc transporters by forskolin in human trophoblast BeWo cells

Reproductive Toxicology
Nagayoshi AsanoYoshiteru Watanabe

Abstract

During pregnancy, the zinc level in fetal serum is up to two-fold higher than that in the maternal serum at the end of pregnancy, but the mechanism of zinc release from the placenta into fetal circulation is not well understood. In this study, we determined the expression profiles of zinc transporters in human trophoblast BeWo cells, a representative human trophoblast cell line. Zn transporter 1-8 (ZnT1-8), Zrt/IRT-like protein 1 and Zn transporter-like transporter 1 were detected in BeWo cells by reverse transcription-polymerase chain reaction. Forskolin (FK) is a representative inducer of differentiation of BeWo cells cytotrophoblast into syncytiotrophoblast. Treatment of BeWo cells with FK resulted in morphological changes of BeWo cells into syncytiotrophoblast cells and secretion of human chorionic gonadotropin, which is a characteristic of syncytiotrophoblast cells. Treatment of BeWo cells with FK elevated ZnT1, 2 and 4 mRNA levels. These data about expression profiles of ZnTs may be useful for further investigation of placental biology.

References

Jun 1, 1992·Journal of Cellular Physiology·N Aslam, H J McArdle
Mar 19, 1991·Biochimica Et Biophysica Acta·A Mas, B Sarkar
Feb 1, 1990·Experimental Cell Research·B WiceA L Schwartz
Jul 1, 1984·American Journal of Obstetrics and Gynecology·A W ZimmermanS L Kunkel
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·R D PalmiterS D Findley
Feb 17, 2000·International Journal of Pharmaceutics·N Utoguchi, K L Audus
Jun 6, 2000·Advanced Drug Delivery Reviews·G T KnippM J Soares
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Giulia RanaldiChiara Murgia
Dec 11, 2002·European Journal of Biochemistry·Masuo KondohMasao Sato
Dec 25, 2002·The Tohoku Journal of Experimental Medicine·Masao Sato, Masuo Kondoh
May 16, 2003·Pflügers Archiv : European journal of physiology·Richard D Palmiter, Liping Huang
Oct 12, 2004·Toxicology Letters·Nagayoshi AsanoYoshiteru Watanabe

❮ Previous
Next ❯

Citations

Aug 2, 2012·Nutrients·Carmen Marino Donangelo, Janet C King
Mar 5, 2019·Metallomics : Integrated Biometal Science·Marco RoversoSilvia Visentin
Oct 1, 2019·Biology of Reproduction·Enoch Appiah Adu-GyamfiYing-Xiong Wang
Dec 15, 2020·Fetal and Pediatric Pathology·Özge Yücel ÇelikDilek Uygur

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.