Induction properties of a transiently transfected glucocorticoid-responsive gene vary with glucocorticoid receptor concentration.

The Journal of Biological Chemistry
D SzaparyS S Simons

Abstract

Transient transfections of steroid receptors have yielded much of the data used to construct the current models of steroid hormone action. These experiments invariably examine the ability of receptors to regulate transcription when occupied by saturating concentrations of steroid. We now report that other induction properties of a transiently transfected gene are not constant but vary with the concentration of transiently transfected glucocorticoid receptors. Thus, the percentage of maximal induction seen with subsaturating concentrations of glucocorticoid could be dramatically increased, and an antiglucocorticoid could be converted into a partial glucocorticoid, simply by increasing the concentration of glucocorticoid receptors. This behavior was observed in HeLa cells, containing endogenous receptors, or in CV-1 cells, containing almost no endogenous receptor, with either homologous or heterologous receptors. These increases were relatively insensitive to the concentration of reporter gene, suggesting the titration of some transcription factor(s) involved in regulating the position of the glucocorticoid dose-response curve and the agonist activity of an antiglucocorticoid. This property of transfected glucocorticoid receptors...Continue Reading

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Citations

May 28, 2003·Trends in Pharmacological Sciences·S Stoney Simons
Feb 13, 2003·Molecular and Cellular Endocrinology·Shiyou Chen, S Stoney Simons
Jan 12, 2000·Molecular and Cellular Endocrinology·Y Huang, S S Simons
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·A M KayeD Somjen
Sep 23, 1998·The Journal of Steroid Biochemistry and Molecular Biology·V G ThackrayS K Nordeen
Aug 27, 1998·The Journal of Steroid Biochemistry and Molecular Biology·D A JacksonS S Simons
Apr 3, 2001·European Journal of Clinical Investigation·C M Bamberger, H M Schulte
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Karen M OngCarson C Chow
Nov 28, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·K C FalknerR A Prough
Jul 18, 2012·Molecular and Cellular Biology·Timothy E ReddyRichard M Myers
Jan 31, 2007·Molecular and Cellular Biology·Sebastiaan H MeijsingKeith R Yamamoto
Aug 21, 2002·Biological & Pharmaceutical Bulletin·Kiyoshi TanigawaKoichi Ikizawa
Jun 28, 2008·Molecular and Cellular Endocrinology·Yunguang SunS Stoney Simons
Jul 16, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Stoney Simons
Sep 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Qi WangS Stoney Simons
May 26, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Yuli KimS Stoney Simons
Jan 1, 2014·Molecular and Cellular Endocrinology·Dong-Hee HanSehyung Cho
Jun 12, 2016·Pharmacology & Therapeutics·Janet P HapgoodJohnson M Moliki
Sep 29, 2005·American Journal of Physiology. Renal Physiology·Christie P ThomasHemender S Vats
Jan 29, 2002·The Journal of Biological Chemistry·Sunil KaulS Stoney Simons

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