PMID: 6165299Jan 1, 1980Paper

Induction, purification, and properties of 2'5' oligoadenylate synthetase

Annals of the New York Academy of Sciences
L A Ball

Abstract

The results presented above relate to several aspects of the 2-5A system. A simple but insensitive radiochemical assay fro 2-5A and its synthetase is described. Progress towards the molecular characterization of the synthetase suggested that it is composed of a single 56,000 dalton polypeptide chain that is synthesized in response to IF treatment. Degradation of 2-5A occurs at the same rapid rate in extracts of IF-treated and untreated chick cells. However, this breakdown can be inhibited by its end-product, 5'AMP, or by the activated synthetase which can further elongate 2-5A and thereby protect it from degradation. The direction of elongation is from the 5' to the 2' terminus. Molecules other than 2-5A can act as substrates fro 2'-adenylation by the activated synthetase. These include some dinucleoside monophosphates, ADP-ribose and NAD+, and methylene-bridged analogues of ATP. The methylene-bridged analogues of 2-5A that are synthesized in the latter case retain some of the biological activity of authentic 2-5A, indicating that cleavage of the 5'-terminal phosphate group(s) is not involved in the mechanism of nuclease activation by 2-5A.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A G HovanessianL Montagnier
Sep 1, 1979·FEBS Letters·B R WilliamsI M Kerr
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A KimchiM Revel
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A SchmidtM Revel
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I M Kerr, R E Brown
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L A Ball, C N White
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P J FarrellP Lengyel
Jan 1, 1978·Annual Review of Biochemistry·M Revel, Y Groner

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Citations

Jan 1, 1984·Neurosurgical Review·M Nagai, T Arai
Oct 18, 1983·Biochimica Et Biophysica Acta·T J BorelliJ M Wu
Jun 12, 1998·Pharmacology & Therapeutics·M R Player, P F Torrence
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Etienne-SmekensJ E Dumont
Sep 1, 1982·European Journal of Biochemistry·M Verhaegen-Lewalle, J Content
Jan 30, 1984·FEBS Letters·S VilcekM Jurovcík
Jan 1, 1989·Annals of the New York Academy of Sciences·D OrlicC Sandoval
Jun 27, 2015·BMC Biochemistry·Jesper Buchhave PoulsenPia Møller Martensen

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