Industrial Genotoxicology Group collaborative trial to investigate cell cycle parameters in human lymphocyte cytogenetics studies

Mutagenesis
L HendersonE J van de Waart

Abstract

Human lymphocyte cultures have been used for many years for assessing the in vitro clastogenic potential of test substances. In these assays the harvest time should be based on the cell cycle time in order to ensure that cells are sampled at an appropriate time for the detection of clastogenic effects. The sources of variation in the cell cycle time in routine cytogenetic assays have not been well studied. Consequently 13 laboratories, all members of the Industrial Genotoxicology Group, participated in a collaborative study to measure the variation in cell cycle time in cultured human peripheral blood lymphocytes under various conditions. The study was performed in two phases, spaced 6 months apart. The average generation time (AGT) was measured by the incorporation of bromodeoxyuridine. Very similar AGTs were found in the presence and absence of S9 mix. The mean AGT (mean of four donors) in each laboratory varied from 11.2 to 17.1 h, indicating there is significant variability in cell cycle times of human peripheral blood lymphocytes between laboratories. There was greater variation between laboratories than within laboratories. A comparison of AGT values at 72 h performed in experiments at least 6 months apart indicated good ...Continue Reading

Citations

Dec 18, 2001·Teratogenesis, Carcinogenesis, and Mutagenesis·R V BurimC S Takahashi
Jun 5, 2002·Molecular and Cellular Endocrinology·O L ClowJ S Fleming
Jun 8, 2013·Bioprocess and Biosystems Engineering·Joy SarkarKrishnendu Acharya
Oct 18, 2011·Journal of Hazardous Materials·Manosij GhoshAnita Mukherjee
Mar 22, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sonali SinhaAnita Mukherjee

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