Industrial Whey Utilization as a Medium Supplement for Biphasic Growth and Bacteriocin Production by Probiotic Lactobacillus casei LA-1

Probiotics and Antimicrobial Proteins
Mukesh KumarAbhijit Ganguli

Abstract

The ability of probiotic Lactobacillus casei LA-1 for bacteriocin production using industrial by-products, such as whey, as supplement in growth medium has been demonstrated for the first time. Whey was investigated as a sole carbon source in cooperation with other components to substitute expensive nutrients as MRS for economical bacteriocin production. Industrial whey-supplemented MRS medium was then selected as to determine the effect of four variables (temperature, initial pH, incubation time, and whey concentration) by response surface methodology on bacteriocin production. Statistical analysis of results showed that two variables have a significant effect on bacteriocin production. Response surface data showed maximum bacteriocin production of 6,132.33 AU/mL at an initial pH of 7.12, temperature 34.29 °C, and whey concentration 13.74 g/L. The production of bacteriocin started during the exponential growth phase, reaching maximum values at stationary phase, and a biphasic growth and production pattern was observed. Our current work demonstrates that this approach of utilization of whey as substitution in costly medium as MRS has great promise for cost reduction in industry for the production of novel biological metabolic p...Continue Reading

References

Feb 3, 1999·Annals of the New York Academy of Sciences·H KimuraA Ishizaki
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Oct 11, 2001·Biotechnology and Applied Biochemistry·S H Flôres, R M Alegre
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Jul 29, 2003·Applied Microbiology and Biotechnology·A S Motta, A Brandelli

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Citations

Dec 21, 2012·Microbiology and Immunology·Alok KumarAbhijit Ganguli
May 11, 2018·Critical Reviews in Food Science and Nutrition·Thiago SidooskiLisiane Fernandes de Carvalho
Dec 19, 2018·Frontiers in Microbiology·Carolina Gutiérrez-CortésSvetoslav Dimitrov Todorov

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