PMID: 9428703Jan 15, 1998Paper

Inefficient repair of RNA x DNA hybrids

European Journal of Biochemistry
A S Kamath-LoebL A Loeb

Abstract

RNA x DNA hybrids are commonly observed during normal biological processes. We tested the ability of three DNA-repair enzymes to remove lesions from the DNA strand of RNA x DNA heteroduplexes. Three nucleotide analogs, 5-hydroxy-2'-deoxycytidine triphosphate, 8-oxo-2'-deoxyguanosine triphosphate, and O6-methyl-2'-deoxyguanosine triphosphate, representative of lesions generated by oxygen damage and methylating agents, were incorporated into the DNA strand synthesized using either a DNA or RNA template. The extended DNA x DNA and RNA x DNA hybrids were used as substrates for bacterial formamidopyrimidine-DNA glycosylase, Nth protein (endonuclease III) and O6-methylguanine-DNA methyltransferase. We show that all three lesions are readily cleaved from the DNA strand of a DNA x DNA duplex but are relatively resistant to cleavage when present in the DNA strand of an RNA x DNA hybrid. Our in vitro studies suggest that damaged DNA in RNA x DNA hybrids is less likely to be repaired in vivo.

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Citations

Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Sep 12, 2001·Bioorganic & Medicinal Chemistry Letters·J C ShielsP H Bolton
Jan 11, 2000·AIDS Research and Human Retroviruses·L A Loeb, J I Mullins
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L A LoebJ I Mullins
Oct 25, 2014·Viruses·Pinar Iyidogan, Karen S Anderson
Jan 15, 2005·Virus Research·Robert A SmithBradley D Preston
Nov 30, 2002·The Journal of Biological Chemistry·Stephane PrietJosephine Sire
May 17, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Richard Daifuku
Apr 27, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J CuiP K Liu

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