Inertial lift enhanced phase partitioning for continuous microfluidic surface energy based sorting of particles

Lab on a Chip
Vahidreza Parichehreh, Palaniappan Sethu

Abstract

A new microfluidics technique that exploits the selectivity of phase partitioning and high-speed focusing capabilities of the inertial effects in flow was developed for continuous label-free sorting of particles and cells. Separations were accomplished by introducing particles at the interface of polyethylene glycol (PEG) and dextran (DEX) phases in rectangular high aspect-ratio microfluidic channels and allowing them to partition to energetically favorable locations within the PEG phase, DEX phase or interface at the center of the microchannel. Separation of partitioned particles was further enhanced via inertial lift forces that develop in high aspect-ratio microchannels that move particles to equilibrium positions close to the outer wall. Combining phase partitioning with inertial focusing ensures selectivity is possible using phase partitioning with sufficient throughput (at least an order of magnitude greater than phase partitioning alone) for application in the clinical and research setting. Using this system we accomplished separation of 15 μm polystyrene (PS) particles from 1-20 μm polymethylmethacrylate (PMMA) particles. Results confirm the feasibility of separation based on phase partitioning and enhancement of separa...Continue Reading

References

Nov 10, 2001·Electrophoresis·M BergerR H Austin
Nov 1, 1962·Experimental Cell Research·P A ALBERTSSON, G D BAIRD
Dec 4, 2003·Nature·M P MacDonaldK Dholakia
Dec 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Josef JancaTatiana G Evseeva
Mar 31, 2004·Lab on a Chip·Andreas NilssonThomas Laurell
Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Oct 2, 2004·Biotechnology and Bioengineering·Masumi YamadaMinoru Seki
Oct 9, 2004·Lab on a Chip·Jitae KimMarc J Madou
Jul 12, 2005·Annual Review of Biomedical Engineering·Mehmet Toner, Daniel Irimia
Sep 19, 2006·Journal of Separation Science·S Kim Ratanathanawongs Williams, Dean Lee
Jun 2, 2007·Acta Biomaterialia·Subra Suresh
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Pu ChenBi-Feng Liu
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dino Di CarloMehmet Toner
Jul 26, 2008·Nature Nanotechnology·Sarah E CrossJames K Gimzewski
Oct 22, 2008·Biomedical Microdevices·Jeffrey R Soohoo, Glenn M Walker
Feb 17, 2009·Analytica Chimica Acta·Barbara RodaAldo Roda
Apr 14, 2009·Cytometry. Part B, Clinical Cytometry·Barbara RodaGian Paolo Bagnara
Sep 22, 2009·The Analyst·Masatoshi TsukamotoEiichi Tamiya
Oct 14, 2009·Lab on a Chip·Dino Di Carlo
Jan 5, 2010·Mechanics Research Communications·Hideaki Tsutsui, Chih-Ming Ho
Jul 1, 2009·New Journal of Physics·Aman RussomMehmet Toner
Sep 25, 2010·Biomedical Microdevices·Harm A NieuwstadtJoseph L Bull
Jan 29, 2011·Lab on a Chip·Soojung Claire HurDino Di Carlo

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Citations

Jan 12, 2013·Lab on a Chip·Vahidreza ParichehrehPalaniappan Sethu
Jul 17, 2012·Methods : a Companion to Methods in Enzymology·Julien AutebertJean-Louis Viovy
Apr 4, 2015·Analytical Chemistry·Xinyu Lu, Xiangchun Xuan
Aug 30, 2018·Biomedical Microdevices·Yuxi Sun, Palaniappan Sethu

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