Inertial Microfluidic Syringe Cell Concentrator

Analytical Chemistry
Nan XiangZhonghua Ni

Abstract

Low-cost, easy-to-use cell concentration tools are in urgent demand for biomedical diagnosis in resource-poor settings. Herein, we propose a novel inertial microfluidic syringe cell (IMSC) concentrator that employs inertial focusing to increase cell concentration through ordering the cell and removing the cell-free fluid. A three-part structure, consisting of a cap-shaped upper housing, a circular gasket, and a lower housing with a spiral channel, is adopted for simple fabricating and assembling, which enables the seamless translation of our IMSC concentrator into commercial outcomes without additional redesigning. The performance characterization indicates that our IMSC concentrator is capable of processing samples with different initial concentrations over a broad flow rate range. The satisfactory concentration performances over a broad driving flow rate range make it possible for our IMSC concentrator to be driven by pushing the syringe with single hand. Finally, pollen particles and MCF-7 cells are successfully concentrated at a high throughput of 3.0 mL/min (up to 4.2 × 107 counts/mL) under the hand-powered drive. We envision wide applications of our IMSC concentrator as "centrifugation on a syringe tip" to various cell co...Continue Reading

References

Dec 24, 2005·Lab on a Chip·Nicole Pamme
Jul 13, 2006·Annual Review of Biomedical Engineering·Joel Voldman
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dino Di CarloMehmet Toner
Oct 1, 2009·Lab on a Chip·Sathyakumar S KuntaegowdanahalliIan Papautsky
Oct 14, 2009·Lab on a Chip·Dino Di Carlo
Apr 27, 2010·Analytical and Bioanalytical Chemistry·Daniel R GossettDino Di Carlo
Jun 1, 2010·Lab on a Chip·Robert GorkinYoon-Kyoung Cho
Jan 29, 2011·Lab on a Chip·Soojung Claire HurDino Di Carlo
Aug 5, 2011·The American Journal of Tropical Medicine and Hygiene·Jocelyn BrownRebecca Richards-Kortum
Oct 19, 2011·The Analyst·Jana C JokerstCharles S Henry
Nov 1, 2011·Applied and Environmental Microbiology·Brandon W PetersonHenk J Busscher
Mar 29, 2012·Physics of Fluids·Joseph M Martel, Mehmet Toner
Feb 6, 2013·Lab on a Chip·Albert J MachDino Di Carlo
Jan 10, 2014·Biomicrofluidics·A KarimiA M Ardekani
Jan 10, 2014·Biomicrofluidics·Nivedita Nivedita, Ian Papautsky
Jun 11, 2014·Lab on a Chip·Hamed AminiDino Di Carlo
Jun 3, 2015·Chemical Society Reviews·O StrohmeierN Paust
Jun 11, 2015·Scientific Reports·Joseph M MartelMehmet Toner
Jul 15, 2015·Scientific Reports·Majid Ebrahimi WarkianiJongyoon Han
Jul 22, 2015·Annual Review of Biomedical Engineering·Majid Ebrahimi WarkianiJongyoon Han
Aug 21, 2015·Annual Review of Biomedical Engineering·Francis CuiAnubhav Tripathi
Nov 17, 2015·Small·Byeongyeon Kim, Sungyoung Choi
Nov 20, 2015·Lab on a Chip·Jun ZhangWeihua Li
Dec 19, 2015·Nature Protocols·Majid Ebrahimi WarkianiChwee Teck Lim
Jan 23, 2016·Lab on a Chip·Chao LiuGuoqing Hu
Jul 6, 2016·Lab on a Chip·Mehdi RafeieMajid Ebrahimi Warkiani
Jul 29, 2017·Scientific Reports·Taehong KwonJongyoon Han
Oct 17, 2017·Scientific Reports·Zi-Long ZhaoYi Min Xie
Mar 2, 2018·Analytical Chemistry·Xinjie ZhangZhonghua Ni

❮ Previous
Next ❯

Citations

Nov 30, 2018·Electrophoresis·Liang-Liang FanLiang Zhao
Jan 11, 2019·Electrophoresis·Xinjie ZhangNan Xiang
Feb 23, 2019·Biotechnology Journal·Reza MoloudiMay Win Naing
May 6, 2020·Annals of Biomedical Engineering·Jung Y Han, Don L DeVoe
Apr 26, 2020·Micromachines·Asma MihandoustMajid Ebrahimi Warkiani
Dec 7, 2018·Analytical Chemistry·Daniel Stoecklein, Dino Di Carlo
Nov 27, 2019·Analytical Chemistry·Ling XiaGongke Li
Oct 29, 2021·Frontiers in Bioengineering and Biotechnology·Han ShanZeyu Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.