Inexpensive handheld device for the construction of high-density nucleic acid arrays

BioTechniques
M SchummerL Hood

Abstract

We report an easy-to-use, 384-pin handheld arraying and replicating device (ARD) for constructing high-density replicas of nucleic acids and E. coli transformants. We have modified an existing 384-pin tool to include a novel guide system to ensure vertical pin movement and enhance reproducibility. An asymmetric rectangular multiplexing frame is designed to increase the array density to 1536 dots on a standard microplate-size nylon membrane and to reduce the time and effort involved in producing array replicas. Our initial studies used the ARD to construct 1536-dot arrays of ovarian cDNA clones. We have hybridized these arrays with 32P-labeled probes, which resulted in distinctive signals for either visual interpretation or semiautomatic spot detection and signal integration.

Citations

Jan 8, 2003·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Farid E Ahmed
Oct 16, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stephen S RobertsSrinivasa R Nagalla
Oct 18, 2000·Biochemical and Biophysical Research Communications·N KhannaN Singh
Dec 28, 1999·Genetic Analysis : Biomolecular Engineering·P S NelsonL Hood
Aug 13, 2003·Plant Physiology·Fábio T S NogueiraPaulo Arruda

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