Infection- and procedure-dependent effects on pulmonary gene expression in the early phase of influenza A virus infection in mice

BMC Microbiology
Matthias PreusseFrank Pessler

Abstract

Investigating the host response in the early stage of influenza A virus (IAV) infection is of considerable interest. However, it is conceivable that effects due to the anesthesia and/or intranasal infection procedure might introduce artifacts. We therefore aimed to evaluate the effects of anesthesia and/or intranasal infection on transcription of selected pulmonary mRNAs in two inbred mouse strains with differential susceptibility to IAV infection. DBA/2J and C57BL/6J mice were evaluated in a time course experiment in which lung tissue was sampled after 6, 12, 18, 24, 48 and 120 h. After anesthesia with ketamine and xylazine, a suspension of mouse-adapted IAV strain PR8_Mun in 20 μl sterile buffer, or 20 μl sterile buffer only, was instilled intranasally. The mice receiving anesthesia and PBS only were designated the "mock treatment" group. Pulmonary expression of 10 host mRNAs (Fos, Retnla, Irg1, Il6, Il1b, Cxcl10, Stat1, Ifng, Ifnl2, and Mx1) and viral hemagglutinin (HA) mRNA were determined at the designated time points. As expected, weight loss and viral replication were greater in the DBA/2J strain (which is more susceptible to IAV infection). Four mRNAs (Retnla, Irg1, Il6, and Cxcl10) were procedure-dependently regulated ...Continue Reading

References

Oct 1, 1988·Molecular and Cellular Biology·P StaeheliO Haller
Aug 26, 1998·Anesthesia and Analgesia·L RoytblatA Duvdenani
Sep 4, 1999·Anesthesia and Analgesia·T KawasakiA Shigematsu
Mar 7, 2000·Intervirology·C M CebullaD D Sedmak
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lidia MichalecSanjiv Sur
Aug 7, 2004·Cell Communication and Signaling : CCS·Andrew C DudleyAlicia Jenkins
Nov 19, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Daniel DegrandiKlaus Pfeffer

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Citations

Jul 3, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Tomás CasanovaMutien-Marie Garigliany
May 15, 2015·Annual Review of Nutrition·Thekla CordesKarsten Hiller
Apr 17, 2015·Poultry Science·Hyun-Jun JangMi Na Park
May 8, 2018·Tissue Engineering. Part a·Rudra BhowmickHeather Gappa-Fahlenkamp
Nov 15, 2018·Immunology and Cell Biology·Xiao-Hua YuChao-Ke Tang
Feb 13, 2020·Reproductive Sciences·Ratana Lim, Martha Lappas
May 18, 2016·The Journal of Biological Chemistry·Thekla CordesChristian M Metallo
Jun 28, 2018·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Heike KollmusKlaus Schughart
Jul 1, 2020·Cellular & Molecular Immunology·Runliu WuRui Kang
Jan 31, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zhongjie YiTimothy R Billiar
Dec 10, 2020·Current Opinion in Biotechnology·Thekla Cordes, Christian M Metallo

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Methods Mentioned

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