Infection and integration of high-risk human papillomavirus in HPV-associated cancer cells

Medical Oncology
Chu-Yi LiuRu-Gang Zhong

Abstract

High-risk human papillomaviruses (HPV) have been associated with many human cancers in clinical studies. Integration of HPV into the human genome is a suspected etiological factor in the induction of some HPV-associated cancers. The characteristics of HPV integration in certain HPV-integrated cancer cells remain unclear. In this study, ten HPV-associated carcinoma cell lines were evaluated for the presence, genotype, and integration status of HPV by nested polymerase chain reaction. The HPV genome did not insert in the genome of a mammary cancer cell line (MCF7), adrenal neuroblastoma cell line (NH-6), or three esophageal carcinoma cell lines (KYSE150, KYSE450 and KYSE140). HPV type 18 DNA did infect cell lines of tongue cancer (Tca83), hepatocellular carcinoma (Hep G2), and lung carcinoma (A549), but the HPV type 18 genes were not transcribed into mRNA. However, HPV type 18 integrated into the genomes of the esophageal carcinoma cell lines EC9706 and EC109, and the integration sites for both cell lines were in loci 8q24, which is a gene desert area adjacent to fragile sites. We speculate that HPV transcripts are more likely to integrate near highly susceptible fragile sites. This study suggests that HPV integration is still a ...Continue Reading

References

May 18, 2004·International Journal of Cancer. Journal International Du Cancer·Camille C Rose RaginSusanne M Gollin
May 5, 2006·Advances in Dental Research·J Palefsky
Jul 14, 2007·Disease Markers·F Xavier Bosch, Silvia de Sanjosé
Aug 18, 2009·Public Health Genomics·Robert D BurkKoenraad Van Doorslaer
Aug 20, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Mine S CicekStephen N Thibodeau
Jun 4, 2010·Gynecologic Oncology·John T SchillerRhonda C Kines
Dec 7, 2010·International Journal of Hygiene and Environmental Health·Qing-Ying ZhangWilliam W Au
Nov 11, 2011·World Journal of Gastroenterology : WJG·Ke ZhangYi Zeng
Jan 6, 2012·Journal of Oncology·E Guzmán-OleaV Madrid-Marina
Mar 28, 2012·Pathology Oncology Research : POR·Clorinda AnnunziataMaria Lina Tornesello
Jun 23, 2012·Current Opinion in Virology·Guillaume FallotMarie-Annick Buendia
Jul 5, 2012·PloS One·Martina SchmitzMatthias Dürst
Mar 28, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J BetiolL Sichero
Sep 7, 2013·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·John T Schiller, Douglas R Lowy
Sep 7, 2013·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Chien-Jen ChenSan-Lin You
Sep 24, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Lorenz K ThomasMiriam Reuschenbach
Feb 14, 2014·Carcinogenesis·Camille RaginEmanuela Taioli
Apr 17, 2014·Oncology Reports·Hilda Jiménez-WencesGloria Fernández-Tilapa
Sep 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Somayeh JalilvandRasool Hamkar
Oct 16, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Kan ZhaiHuan-Zhong Shi

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