Infection assays in Arabidopsis reveal candidate effectors from the poplar rust fungus that promote susceptibility to bacteria and oomycete pathogens

Molecular Plant Pathology
Hugo GermainArmand Séguin

Abstract

Fungi of the Pucciniales order cause rust diseases which, altogether, affect thousands of plant species worldwide and pose a major threat to several crops. How rust effectors-virulence proteins delivered into infected tissues to modulate host functions-contribute to pathogen virulence remains poorly understood. Melampsora larici-populina is a devastating and widespread rust pathogen of poplar, and its genome encodes 1184 identified small secreted proteins that could potentially act as effectors. Here, following specific criteria, we selected 16 candidate effector proteins and characterized their virulence activities and subcellular localizations in the leaf cells of Arabidopsis thaliana. Infection assays using bacterial (Pseudomonas syringae) and oomycete (Hyaloperonospora arabidopsidis) pathogens revealed subsets of candidate effectors that enhanced or decreased pathogen leaf colonization. Confocal imaging of green fluorescent protein-tagged candidate effectors constitutively expressed in stable transgenic plants revealed that some protein fusions specifically accumulate in nuclei, chloroplasts, plasmodesmata and punctate cytosolic structures. Altogether, our analysis suggests that rust fungal candidate effectors target distin...Continue Reading

References

Apr 25, 2017·Phytopathology·Bret Cooper, Kimberly B Campbell
Mar 1, 2018·Frontiers in Microbiology·Maíra de Freitas PereiraMartina Peter
Oct 31, 2017·The New Phytologist·Cecilia Cheval, Christine Faulkner
Dec 17, 2018·The New Phytologist·Cécile LorrainSébastien Duplessis
Dec 5, 2019·Phytopathology·Guus Bakkeren, Les J Szabo
Feb 25, 2020·Journal of Experimental Botany·Aliyeh RasoolizadehRichard R Bélanger
Jan 12, 2020·BMC Genomics·Karen Cristine Gonçalves Dos SantosHugo Germain
Dec 28, 2019·Pathogens·Matthias KretschmerJames Kronstad
Sep 15, 2020·World Journal of Microbiology & Biotechnology·Rajdeep JaswalT R Sharma
Oct 5, 2018·Scientific Reports·Md Bulbul AhmedHugo Germain
Aug 9, 2020·Molecular Plant Pathology·Claire Kanja, Kim E Hammond-Kosack

Citations

Dec 30, 1998·Trends in Biochemical Sciences·E A Van der Biezen, J D Jones
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jul 19, 2001·Nature·Jeffery L Dangl, J D Jones
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Jan 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maike C RentelBrian J Staskawicz
Apr 22, 2008·Annual Review of Phytopathology·Vera Göhre, S Robatzek
Jan 10, 2009·Molecular Plant-microbe Interactions : MPMI·S A HogenhoutSophien Kamoun
Oct 31, 2009·The Plant Journal : for Cell and Molecular Biology·Gregory J LawrenceJeffrey G Ellis
Sep 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·S SchornackEdgar Huitema
Jan 5, 2011·PLoS Genetics·Hugo GermainXin Li
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sébastien DuplessisFrancis Martin
Sep 15, 2011·The Plant Journal : for Cell and Molecular Biology·Marie-Cecile CaillaudJonathan D G Jones
Jan 1, 2011·Journal of Pathogens·Stéphane HacquardSébastien Duplessis
Jun 5, 2012·Current Opinion in Plant Biology·Maryam RafiqiPeter N Dodds
Dec 12, 2012·Cold Spring Harbor Symposia on Quantitative Biology·Joe WinSophien Kamoun
Aug 21, 2013·Science·Jeffery L DanglBrian J Staskawicz
Oct 17, 2013·Nature Reviews. Microbiology·Martha C Giraldo, Barbara Valent
Sep 6, 2014·Frontiers in Plant Science·Benjamin PetreSébastien Duplessis
Dec 17, 2014·Virulence·Prateek ChaudhariHugo Germain
Feb 5, 2015·Molecular Plant-microbe Interactions : MPMI·Benjamin PetreSophien Kamoun
Oct 2, 2015·Cellular Microbiology·Benjamin PetreSophien Kamoun
Nov 25, 2015·Current Opinion in Microbiology·Madiha KhanDarrell Desveaux
Dec 30, 2015·Molecular Plant-microbe Interactions : MPMI·Oliver X DongHugo Germain

Related Concepts

Allergy Testing Poplar
poplar antigen
Plant Immune Response
Crops, Agricultural
Genome
Arabidopsis
Pathogenic Organism
Plants, Transgenic
Cell Nucleus
Chloroplasts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.