Infection by Cx43 adenovirus increased chemotherapy sensitivity in human gastric cancer BGC-823 cells: not involving in induction of cell apoptosis

Gene
Dan LiuXufeng Zhang

Abstract

There is a lower basal expression of Connexin43 (Cx43) in human gastric cancer BGC-823 cells. In the present study, BGC-823 cells were transfected with recombinant Cx43 adenovirus plasmid vector, and we explored the influences of Cx43 expression on cell proliferation, chemo-sensitivity, colony forming ability, invasion ability and apoptosis. Moreover, we also determined the expression of Pgp, Cx43, as well as apoptosis-related proteins (bcl-2, bax, caspase3 and caspase 9). MTT assay was performed to determine the proliferation of BGC-823 cells before and after Cx43 transfection. The influences of Cx43 infection on sensitivity of chemotherapy (including Doxorubicin, fluorouracil, oxaliplatin) were detected by MTT assay. Expression levels of Pgp, Cx43, as well as apoptosis-related proteins (bcl-2, bax, caspase-3 and caspase-9) in BGC-823 cells were determined by Western blotting analysis before and after the infection with Cx43 adenovirus. MDR expression was determined by RT-PCR before and after Cx43 infection. Invasive ability was detected by invasion chamber. Influence of Cx43 adenovirus infection on apoptosis of BGC-823 cells was determined by flow cytometry. After infection by Cx43 adenovirus, colony forming rate and invasive...Continue Reading

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Citations

Aug 16, 2016·Journal of Cancer Research and Clinical Oncology·Lauana Greicy Tonon LemosRaquel Ciuvalschi Maia
Sep 6, 2020·Journal of Cellular and Molecular Medicine·Xiaofei QuWeijian Guo
Sep 29, 2019·World Journal of Gastroenterology : WJG·Huan LiXiao-Ming Liu
Mar 1, 2019·Oncogene·Trond AasenArantxa Tabernero
Oct 1, 2021·The Journal of International Medical Research·Shasha LiuXingbin Shen

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