PMID: 9531019Apr 8, 1998Paper

Infection characteristics of rabies virus variants with deletion or insertion in the pseudogene sequence

Journal of Neurovirology
P E CeccaldiH Tsiang

Abstract

We investigated the infection characteristics of recombinant rabies virus variants modified in the pseudogene sequence. Infection of neuronal cell lines by the SAD W9 and SAD V* variants (respectively with deletion or insertion in this sequence) showed no significant differences as compared to the parental strain, the attenuated strain SAD B19, in infection characteristics such as number of infected cells or viral yield. The inoculation of mice by these variants resulted in similar infection patterns and pathogenicity. Stereotaxic inoculation of the different variants into the rat striatum showed that deletion or insertion did not affect the axonal virus spread, nor did insertion of a complete additional transcription unit, that could be expressed in the areas connected to the inoculation site. These results show that the pseudogene sequence is not involved in viral spread and pathogenicity and confirm the availability of this domain for targeting and expression of foreign genes into neurons.

Citations

Dec 31, 2002·Journal of Virological Methods·Ken ichi InoueKinjiro Morimoto
Sep 22, 2011·Virus Research·Peter J WalkerKim R Blasdell
Sep 13, 2005·Lancet·Monique Lafon
Feb 14, 2015·PLoS Pathogens·Peter J WalkerNikos Vasilakis
Jun 20, 2017·PLoS Neglected Tropical Diseases·Elisa EggerbauerConrad M Freuling

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