Infection in mouse peritoneal cavity with a pyrimidine-requiring mutant and naturally occurring Staphylococcus aureus strains.

Journal of Bacteriology
L G MathieuS Sonea

Abstract

The lethal activity of a thymineless mutant of Staphylococcus aureus Wood 46 strain has been compared with that of three naturally occurring strains: parent Wood 46, Smith, and coagulase-negative SA-13. The thymineless mutant and the parent Wood 46 strain showed a sharp decline in culturable units from the peritoneal cavity in the first 4 hr after their injection. After 6 hr, that is, 2 hr before the mice began to die, the number of culturable units of the thymineless mutant was still declining, whereas that of the parent strain increased; for both strains, the number of units was still lower than that of the inoculum. Although the thymineless mutant, unlike the parent strain, was apparently unable to multiply in mouse peritoneal cavity, it killed mice at a similar rate. The highly virulent Smith strain known to multiply rapidly and the avirulent coagulase-negative SA-13 strain were used as additional controls. Under our experimental conditions, death of mice after the injection of the thymineless mutant in the peritoneal cavity did not seem to be due to bacterial multiplication but to toxicity, death being delayed by antitoxin. The pyrimidine-requiring auxotroph we used could be better material than killed bacteria to study so...Continue Reading

References

Jul 23, 1965·Annals of the New York Academy of Sciences·S I Morse
Oct 1, 1966·Journal of Bacteriology·F A Kapral
Dec 1, 1962·Bacteriological Reviews·J E BLAIR
Jan 1, 1963·Annual Review of Microbiology·C PANOS, S J AJL

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Citations

Mar 1, 1974·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·L A Devriese, J Hommez
Jul 25, 2017·Trends in Microbiology·Daniel A PensingerJohn-Demian Sauer

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