Infection of CD4+ T lymphocytes by the human T cell leukemia virus type 1 is mediated by the glucose transporter GLUT-1: evidence using antibodies specific to the receptor's large extracellular domain

Virology
Qingwen JinGhalib Alkhatib

Abstract

To analyze HTLV-1 cytotropism, we developed a highly sensitive vaccinia virus-based assay measuring activation of a reporter gene upon fusion of two distinct cell populations. We used this system in a functional cDNA screening to isolate and confirm that the glucose transporter protein 1 (GLUT-1) is a receptor for HTLV-1. GLUT-1 is a ubiquitously expressed plasma membrane glycoprotein with 12 transmembrane domains and 6 extracellular loops (ECL). We demonstrate for the first time that peptide antibodies (GLUT-IgY) raised in chicken to the large extracellular loop (ECL1) detect GLUT-1 at the cell surface and inhibit envelope (Env)-mediated fusion and infection. Efficient GLUT-IgY staining was detected with peripheral blood CD4(+) lymphocytes purified by positive selection. Further, GLUT-IgY caused efficient inhibition of Env-mediated fusion and infection of CD4(+) T and significantly lower inhibition of CD8(+) T lymphocytes. The specificity of GLUT-IgY antibodies to GLUT-1 was demonstrated by ECL1 peptide competition studies. Grafting ECL1 of GLUT-1 onto the receptor-negative GLUT-3 conferred significant receptor activity. In contrast, grafting ECL1 of GLUT-3 onto GLUT-1 resulted in a significant loss of the receptor activity. T...Continue Reading

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Citations

Jul 1, 2006·Journal of Virology·David GhezOlivier Hermine
Dec 1, 2010·Retrovirology·David GhezOlivier Hermine
Dec 9, 2009·Expert Review of Anti-infective Therapy·Péter BagossiJózsef Tözsér
Feb 2, 2016·Viruses·Jessica L MartinLouis M Mansky
Oct 5, 2012·AIDS Research and Human Retroviruses·Atsushi Jinno-OueHiroo Hoshino
Jun 25, 2008·International Reviews of Immunology·Takeo Ohsugi, Atsushi Koito
Mar 31, 2009·Arquivos de neuro-psiquiatria·Abelardo Q C AraújoMarcus Tulius T Silva
Mar 16, 2017·Frontiers in Molecular Neuroscience·Manuel SchützeDébora M de Miranda
Oct 20, 2020·The Journal of Clinical Investigation·Kenta TezukaIsao Hamaguchi
Apr 3, 2021·PloS One·Md Thosif RazaShah Md Shahik

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