Infection of HeLa cells by avian infectious bronchitis virus is dependent on cell status

Avian Pathology : Journal of the W.V.P.A
H Y ChenH C Chen

Abstract

To investigate the adaptation of avian infectious bronchitis virus (IBV) in a human cell line may be beneficial to understanding the potential mechanisms of coronavirus interspecies infection. The current study addressed the poor replication of IBV in the HeLa human cell line demonstrated in previous reports. We showed that IBV strains M41, H52, H120 and Gray could be propagated in HeLa cells with distinct cytopathic effect. The virus titre in freshly dispersed HeLa cells was 1000-fold higher than in cell monolayers. Trypsin was not the determinant for the viral replication, suggesting that the restriction of IBV replication in HeLa cells is the result of intracellular events rather than the binding to or fusion with host cells. These IBV strains replicated to an average titre of 10(3.4+/-0.2)/0.1 ml median tissue culture infectious doses in freshly dispersed HeLa cells and maintained this titre for the first 12 passages. Then an approximately 10-fold increase (10(4.20+/-0.19)/0.1 ml) occurred in passage 13, which was maintained to passage 16, after which there was another, bigger rise to 10(6.6+/-0.3)/0.1 ml in passage 17. This titre was maintained until passage 24 when the experiment was terminated. The IBV M41 S1 gene was am...Continue Reading

References

Dec 1, 1974·The Journal of General Virology·G Appleyard, H B Maber
Sep 1, 1972·The Journal of General Virology·C H CunninghamK Nazerian
Jan 1, 1983·Journal of Medical Virology·C J BirchI D Gust
Sep 3, 2005·Biochemical and Biophysical Research Communications·Shou Guo FangD X Liu
Mar 16, 2006·Avian Pathology : Journal of the W.V.P.A·Dave Cavanagh
Apr 11, 2006·The Journal of General Virology·Christine WinterGeorg Herrler
May 18, 2006·Science in China. Series C, Life Sciences·Bo PengAizhen Guo
Apr 1, 2001·Avian Pathology : Journal of the W.V.P.A·D Cavanagh

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Citations

Sep 30, 2020·Pathogens·Shahnas M NajimudeenMohamed Faizal Abdul-Careem
May 29, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jack Chun-Chieh HsuPeter Cresswell

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Datasets Mentioned

BETA
AF3523151

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
ELISA
PCR
glycosylation

Software Mentioned

DNAstar
Meglign

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