PMID: 8963027Sep 1, 1995Paper

Infection of human hepatocyte cell lines with hepatitis C virus in vitro

Journal of Gastroenterology and Hepatology
M TagawaM Omata

Abstract

Hepatocyte related cell lines, namely human embryonic hepatocyte cell line (WRL68) and hepatoblastoma cell line (Hep G2), were tested for their ability to support hepatitis C virus (HCV) replication in vitro. The replicative intermediate of the minus strand HCV RNA was newly transcribed from the inoculated virus, and was stable in WRL68 cells over a period of 62 days. HCV RNA detected in the culture medium at 62 days after infection suggested a long period of virus secretion from this cell line. In Hep G2, transcription of the minus strand HCV RNA was also detected until 39 days after infection, but transcription and secretion of viral progeny could not be demonstrated. Although HCV infection of WRL68 was not high, this cell line might prove to be a useful tool for studying HCV replication in vitro.

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Citations

Apr 4, 2001·Journal of Medical Virology·R GermiE Drouet
Sep 1, 2001·Antiviral Research·R Bartenschlager, V Lohmann
Jan 24, 2002·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·D FavreC Trépo
Jun 21, 2011·Annual Review of Microbiology·Catherine L Murray, Charles M Rice
Dec 14, 2006·Clinics in Liver Disease·Chen Liu
Mar 23, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Reginald F ClaytonArvind H Patel
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
Jan 31, 2002·World Journal of Gastroenterology : WJG·Z Q SongG D Liu
Aug 17, 2004·World Journal of Gastroenterology : WJG·Ying-Song WuMing Li
Oct 30, 2013·Journal of Medicinal Chemistry·Volker Lohmann, Ralf Bartenschlager

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