Infection of Mosquito Cells (C6/36) by Dengue-2 Virus Interferes with Subsequent Infection by Yellow Fever Virus

Vector Borne and Zoonotic Diseases
Emiliana Pereira Abrao, Benedito Antônio Lopes da Fonseca

Abstract

Dengue is one of the most important diseases caused by arboviruses in the world. Yellow fever is another arthropod-borne disease of great importance to public health that is endemic to tropical regions of Africa and the Americas. Both yellow fever and dengue viruses are flaviviruses transmitted by Aedes aegypti mosquitoes, and then, it is reasonable to consider that in a given moment, mosquito cells could be coinfected by both viruses. Therefore, we decided to evaluate if sequential infections of dengue and yellow fever viruses (and vice-versa) in mosquito cells could affect the virus replication patterns. Using immunofluorescence and real-time PCR-based replication assays in Aedes albopictus C6/36 cells with single or sequential infections with both viruses, we demonstrated the occurrence of viral interference, also called superinfection exclusion, between these two viruses. Our results show that this interference pattern is particularly evident when cells were first infected with dengue virus and subsequently with yellow fever virus (YFV). Reduction in dengue virus replication, although to a lower extent, was also observed when C6/36 cells were initially infected with YFV followed by dengue virus infection. Although the impor...Continue Reading

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Sep 18, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christine V F Carrington, Albert J Auguste
Nov 11, 2016·PloS One·Nádia Cristina Pinheiro RodriguesIuri da Costa Leite
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Nov 11, 2020·Viruses·Mathilde LauretiJulio Rodriguez-Andres
Jan 29, 2021·Journal of Travel Medicine·Bethan Cracknell DanielsIlaria Dorigatti

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Methods Mentioned

BETA
optical microscopy

Software Mentioned

Capture

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