PMID: 11899434Mar 20, 2002Paper

Infection of murine keratinocytes with herpes simplex virus type 1 induces the expression of interleukin-10, but not interleukin-1 alpha or tumour necrosis factor-alpha

Immunology
M Zak-PrelichR C McKenzie

Abstract

Herpes simplex virus (HSV) is known to possess several mechanisms whereby it can evade the normal host immune defences. In this study the expression of the immunosuppressive cytokine, interleukin (IL)-10, was monitored following infection of a murine keratinocyte cell line (PAM-212) and compared with the expression of two proinflammatory cytokines: IL-1 alpha and tumour necrosis factor (TNF)-alpha. The PAM-212 cells were infected at a multiplicity of 0.5 with a clinical isolate of HSV type 1, and the mRNA of the three cytokines was assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) over the following 24 hr. By 12 hr postinfection the amount of IL-10 mRNA had increased significantly to five-fold greater than that found in uninfected cells (P < 0.01), and this elevated level was maintained until at least 24 hr postinfection. In contrast, IL-1 alpha and TNF-alpha mRNAs were not significantly up-regulated by the HSV infection. Immunostaining with an IL-10 monoclonal antibody (mAb) revealed that cytoplasmic IL-10 protein had increased by 6-12 hr postinfection. This quantity was further increased at 24 hr postinfection, when the viral cytopathic effect was apparent. Viral replication was necessary, ...Continue Reading

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Citations

Feb 16, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Barry T Rouse, Shilpa Deshpande Kaistha
Oct 24, 2002·Current Opinion in Hematology·Ernst Holler
Apr 14, 2007·American Journal of Reproductive Immunology : AJRI·Sherie FernandezCharu Kaushic
Jul 16, 2008·Journal of the European Academy of Dermatology and Venereology : JEADV·B LeinweberR R Müllegger
Jan 20, 2011·International Journal of Immunopathology and Pharmacology·G DonnarummaR Serpico

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