Infection of the brown alga Ectocarpus siliculosus by the oomycete Eurychasma dicksonii induces oxidative stress and halogen metabolism

Plant, Cell & Environment
Martina StrittmatterFrithjof C Küpper

Abstract

Pathogens are increasingly being recognized as key evolutionary and ecological drivers in marine ecosystems. Defence mechanisms of seaweeds, however, have mostly been investigated by mimicking infection using elicitors. We have established an experimental pathosystem between the genome brown model seaweed Ectocarpus siliculosus and the oomycete Eurychasma dicksonii as a powerful new tool to investigate algal responses to infection. Using proteomics, we identified 21 algal proteins differentially accumulated in response to Eu. dicksonii infection. These include classical algal stress response proteins such as a manganese superoxide dismutase, heat shock proteins 70 and a vanadium bromoperoxidase. Transcriptional profiling by qPCR confirmed the induction of the latter during infection. The accumulation of hydrogen peroxide was observed at different infection stages via histochemical staining. Inhibitor studies confirmed that the main source of hydrogen peroxide is superoxide converted by superoxide dismutase. Our data give an unprecedented global overview of brown algal responses to pathogen infection, and highlight the importance of oxidative stress and halogen metabolism in these interactions. This suggests overlapping defence ...Continue Reading

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Citations

Oct 13, 2017·Molecular Plant Pathology·Arne SchwelmSigrid Neuhauser
Dec 16, 2017·MSphere·Louisi Souza de OliveiraFabiano L Thompson
Mar 6, 2019·Metallomics : Integrated Biometal Science·Frithjof C Küpper, Carl J Carrano
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Dec 1, 2019·International Journal of Molecular Sciences·Lei TangYunxiang Mao
Apr 25, 2018·Marine Biotechnology·Thillai PunithaJohn Beardall
Nov 25, 2020·Annual Review of Genetics·Susana M Coelho, J Mark Cock
Jun 3, 2021·The New Phytologist·Yacine BadisJ Mark Cock

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Datasets Mentioned

BETA
PXD001458

Methods Mentioned

BETA
PCR
protein folding
electron microscopy

Software Mentioned

REST
Progenesis
[UNK]
Mascot

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