Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays.

Biomedical Microdevices
Ying ZhuJohn Yin

Abstract

The plaque assay has long served as the "gold standard" to measure virus infectivity and test antiviral drugs, but the assay is labor-intensive, lacks sensitivity, uses excessive reagents, and is hard to automate. Recent modification of the assay to exploit flow-enhanced virus spread with quantitative imaging has increased its sensitivity. Here we performed flow-enhanced infection assays in microscale channels, employing passive fluid pumping to inoculate cell monolayers with virus and drive infection spread. Our test of an antiviral drug (5-fluorouracil) against vesicular stomatitis virus infections of BHK cell monolayers yielded a two-fold improvement in sensitivity, relative to the standard assay based on plaque counting. The reduction in scale, simplified fluid handling, image-based quantification, and higher assay sensitivity will enable infection measurements for high-throughput drug screening, sero-conversion testing, and patient-specific diagnosis of viral infections.

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Citations

Jun 16, 2010·Analytical and Bioanalytical Chemistry·Iuliana OitaDebby Mangelings
Dec 23, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Samartha G AnekalJohn Yin
Jun 5, 2013·Biomicrofluidics·Elisa CimettaNicola Elvassore
Apr 22, 2015·Annual Review of Virology·Vyas RamananSangeeta N Bhatia
Oct 1, 2010·Microfluidics and Nanofluidics·Amy L McPherson, Glenn M Walker
Jan 10, 2012·Virology·Andrea Timm, John Yin
Jan 27, 2015·Annual Review of Pathology·Kambez H BenamDonald E Ingber
Sep 29, 2012·Journal of the American Chemical Society·Darija MuharemagicMaxim V Berezovski
Aug 19, 2017·Lab on a Chip·Philip C GachAnup K Singh
Jan 10, 2013·Lab on a Chip·Yun ShuZhi-Ling Zhang
Nov 15, 2018·Micromachines·Jeong A KimGyu Man Kim
Jul 24, 2020·Biomicrofluidics·Qasem Ramadan, Mohammed Zourob
Mar 7, 2021·Pathogens·Buket Baddal, Pasquale Marrazzo
Jun 15, 2021·Frontiers in Cellular and Infection Microbiology·Benjamin O MurrayJennifer L Rohn

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