PMID: 9420229Jan 7, 1998Paper

Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other than AAV type 2

Journal of Virology
E A RutledgeD W Russell

Abstract

Adeno-associated viruses (AAVs) are single-stranded dependent parvoviruses being developed as transducing vectors. Although at least five serotypes exist (AAV types 1 to 5 [AAV1 to -5]), only AAV2, AAV3, and AAV4 have been sequenced, and the vectors in use were almost all derived from AAV2. Here we report the cloning and sequencing of a second AAV3 genome and a new AAV serotype designated AAV6 that is related to AAV1. AAV2, AAV3, and AAV6 were 82% identical at the nucleotide sequence level, and AAV4 was 75 to 78% identical to these AAVs. Significant sequence variation was noted in portions of the capsid proteins that presumably are responsible for serotype-specific functions. Vectors produced from AAV3 and AAV6 differed from AAV2 vectors in host range and serologic reactivity. The AAV3 and AAV6 vector serotypes were able to transduce cells in the presence of serum from animals previously exposed to AAV2 vectors. Our results suggest that vectors based on alternative AAV serotypes will have advantages over existing AAV2 vectors, including the transduction of different cell types, and resistance to neutralizing antibodies against AAV2. This could be especially important for gene therapy, as significant immunity against AAV2 exists...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S C Fields-BerryC L Cepko
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·P L ZeitlinW B Guggino
Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Mar 5, 1989·Journal of Molecular Biology·R A Bohenzky, K I Berns
Jan 1, 1987·Molecular and Cellular Biology·R B DuBridgeM P Calos
Dec 1, 1987·Genetics·S Henikoff, M K Eghtedarzadeh
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Nov 1, 1968·American Journal of Epidemiology·N R BlacklowW P Rowe
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·N R CozzarelliA Kornberg
Feb 1, 1967·Journal of Virology·W P ParksH D Mayor
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M D HogganW P Rowe
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Feb 1, 1983·Journal of Virology·A SrivastavaK I Berns
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·D W RussellI E Alexander
May 1, 1996·Journal of Virology·S A AfioneT R Flotte
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·R M LindenK I Berns
Oct 29, 1997·Journal of Virology·E A Rutledge, D W Russell

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Citations

Feb 19, 2000·Gene Therapy·P E Monahan, R J Samulski
Mar 30, 2000·The Journal of Gene Medicine·R O Snyder
Mar 7, 2002·Current Opinion in Pediatrics·Christopher E Walsh
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Qing XieMichael S Chapman
Jan 29, 2003·Journal of Virology·Bernd Hauck, Weidong Xiao
Jul 2, 2003·Gene Therapy·H BüningM Hallek
Sep 23, 2003·Gene Therapy·Charles H ViteJohn H Wolfe
Jul 27, 2004·Nature Medicine·Paul GregorevicJeffrey S Chamberlain
Jun 18, 2005·Cancer Gene Therapy·Chengwen LiRichard Jude Samulski
Jun 15, 2005·Annual Review of Biochemistry·Inder M Verma, Matthew D Weitzman
Aug 1, 2006·Journal of Pediatric Gastroenterology and Nutrition·Guohong ShaoThomas J Sferra
Apr 5, 2007·Nature Protocols·Joshua C GriegerR Jude Samulski
Aug 19, 2007·Journal of Virology·Mariacarmela AlloccaAlberto Auricchio
Mar 15, 2008·The Journal of Gene Medicine·Masaya KonishiToshio Yamashita
Feb 19, 2008·The Journal of Gene Medicine·Corinna LebherzJames M Wilson
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lauren E MaysJames M Wilson
Jun 25, 2010·The Journal of General Virology·Yan LiZhengli Shi
Feb 16, 2011·Human Gene Therapy·J Nicholas Betley, Scott M Sternson
Apr 21, 2011·Expert Opinion on Biological Therapy·Janice Y Chou, Brian C Mansfield
Apr 2, 2011·Nature Protocols·Iram F KhanDavid W Russell
Apr 20, 2012·Journal of Virology·Christie L BellJames M Wilson
Mar 27, 2013·Scientific Reports·Scott BieberGuy L Odom
Jul 19, 2013·Brain : a Journal of Neurology·Atsushi SakaiHidenori Suzuki

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