Infectious laryngotracheitis virus (ILTV) vaccine intake evaluation by detection of virus amplification in feather pulps of vaccinated chickens

Vaccine
Irit DavidsonN Elkin

Abstract

Infectious laryngotracheitis (ILT) is a respiratory disease of poultry caused by an alphaherpesvirus, ILTV. The live vaccine is applied worldwide by drinking water or by the respiratory route, and by the vent application in Israel. No system of direct evaluation of the efficacy of vaccination exists today, except of antibody elicitation, which is an indirect indication of vaccination intake and might happen due to environment exposure. We suggest for the first time an assay for evaluating the accuracy of the vaccination process by spotting the spread of the live vaccine systemically, namely by virus detection in the feather shafts of the vaccinated birds. The feathers are particularly beneficial as they are easy to collect, non-lethal for the bird, therefore advantageous for monitoring purposes. Moreover, the continuous survey of the vaccine virus unveiled the different kinetics of viremia by the different vaccination routes; while after the vent vaccination the systemic viremia peaks during the first week afterwards, after two consecutive vaccine administration by drinking water with 6 day interval, the vireamia peaks only after the second administration. A robust amplification was needed because the vaccine ILTV was present i...Continue Reading

References

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Jan 22, 2009·Avian Pathology : Journal of the W.V.P.A·Ivomar OldoniMaricarmen García
Sep 8, 2009·Letters in Applied Microbiology·Y Hu, I Arsov
Jan 28, 2014·Avian Pathology : Journal of the W.V.P.A·Kimberly R MenendezNathaniel L Tablante

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Citations

Jun 15, 2019·Avian Pathology : Journal of the W.V.P.A·Vishnu KumarSachin Kumar

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