Infectious Sporozoites of Plasmodium berghei Effectively Activate Liver CD8α+ Dendritic Cells

Frontiers in Immunology
Rajesh ParmarSarat Dalai

Abstract

Immunization with radiation-attenuated sporozoites (RAS) shown to confer complete sterile protection against Plasmodia liver-stage (LS) infection that lasts about 6 to 9 months in mice. We have found that the intermittent infectious sporozoite challenge to immune mice following RAS vaccination extends the longevity of sterile protection by maintaining CD8+ T cell memory responses to LS infection. It is reported that CD8α+ dendritic cells (DCs) are involved in the induction of LS-specific CD8+ T cells following RAS or genetically attenuated parasite (GAP) vaccination. In this study, we demonstrate that CD8α+ DCs respond differently to infectious sporozoite or RAS inoculation. The higher accumulation and activation of CD8α+ DCs was seen in the liver in response to infectious sporozoite 72 h postinoculation and found to be associated with higher expression of chemokines (CCL-20 and CCL-21) and type I interferon response via toll-like receptor signaling in liver. Moreover, the infectious sporozoites were found to induce qualitative changes in terms of the increased MHCII expression as well as costimulatory molecules including CD40 on the CD8α+ DCs compared to RAS inoculation. We have also found that infectious sporozoite challenge ...Continue Reading

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Citations

Sep 28, 2020·Parasite Immunology·Kelvin Mokaya AbugaJulius Clemence R Hafalla
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Xiao HeXin-Zhuan Su
Dec 29, 2020·Frontiers in Cellular and Infection Microbiology·Chunmei CaiXiao Yu

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Datasets Mentioned

BETA
SRP129582

Methods Mentioned

BETA
FACS
PCR
RNA-seq
RNA Seq
flow cytometry
As
Well

Software Mentioned

Bowtie
panther
panther pathway analyser
KEGG pathway analyser server
Trimmomatic
GraphPad
TopHat
FlowJo
GraphPad Prism

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